Rad21 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-203
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 575 and the C-terminus (residue 631) human Rad21 homolog using the numbering given in entry NP_006256.1 (GeneID 5885).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Rad21 Antibody
Rad21 Antibody [NB100-203] - Detection of human and mouse Rad21 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, Jurkat, HEK293T, RKO, GaMG, MCF-7, A-549, U2OS, NIH 3T3, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Rad21 antibody NB100-203 used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Rad21 Antibody [NB100-203] - Detection of human Rad21 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-Rad21 antibody NB100-203 (Lot NB100-203-8) used for IP at 6 ug per reaction. Rad21 was also immunoprecipitated by a previous lot of this antibody (lot NB100-203-6). For blotting immunoprecipitated Rad21, rabbit anti-Rad21 recombinant monoclonal antibody NB100-203 was used at 1:1000. Chemiluminescence with an exposure time of 3 minutes.Previous
Applications for Rad21 Antibody
Application
Recommended Usage
Immunoprecipitation
2- 10 ug/mg of lysate
Western Blot
1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Rad21
Alternate Names
double-strand-break repair protein rad21 homolog, hHR21FLJ25655, HR21, KIAA0078RAD21 (S. pombe) homolog, MCD1, Nuclear matrix protein 1, NXP-1, NXP1HRAD21, protein involved in DNA double-strand break repair, RAD21 homolog (S. pombe), SCC1 homolog, SCC1FLJ40596
Gene Symbol
RAD21
Additional Rad21 Products
Product Documents for Rad21 Antibody
Product Specific Notices for Rad21 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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