Rad50 Antibody (JE60-04)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32890
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE60-04
Concentration
1 mg/ml
Product Summary for Rad50 Antibody (JE60-04)
Immunogen
Recombinant protein within Human Rad50 aa 1,162-1,312 / 1,312. (Uniprot: Q92878)
Localization
Nucleus, Chromosome, telomere.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
154 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Rad50 Antibody (JE60-04)
Western Blot: Rad50 Antibody (JE60-04) [NBP3-32890]
Western Blot: Rad50 Antibody (JE60-04) [NBP3-32890] - Western blot analysis of Rad50 on different lysates with Rabbit anti-Rad50 antibody (NBP3-32890) at 1/1,000 dilution.Lane 1: HeLa cell lysate (20 ug/Lane)
Lane 2: K-562 cell lysate (20 ug/Lane)
Lane 3: U-87 MG cell lysate (20 ug/Lane)
Lane 4: U-937 cell lysate (20 ug/Lane)
Lane 5: Caco-2 cell lysate (20 ug/Lane)
Lane 6: Mouse testis tissue lysate (40 ug/Lane)
Lane 7: Rat testis tissue lysate (40 ug/Lane)
Predicted band size: 154 kDa
Observed band size: 154 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32890) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.
Applications for Rad50 Antibody (JE60-04)
Application
Recommended Usage
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Rad50
Alternate Names
DNA repair protein RAD50, EC 3.6, EC 3.6.1.15, EC 3.6.3, EC 3.6.3.27, hRAD50, NBSLD, RAD50 (S. cerevisiae) homolog, RAD50 homolog (S. cerevisiae), RAD502, RAD50-2
Gene Symbol
RAD50
Additional Rad50 Products
Product Documents for Rad50 Antibody (JE60-04)
Product Specific Notices for Rad50 Antibody (JE60-04)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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