Rad50 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47316

Novus Biologicals, part of Bio-Techne
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NBP2-47316
NBP2-47316-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Rad50 Antibodies »

Product Summary for Rad50 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: ELREPQFRDAEEKYREMMIVMRTTELVNKDLDIYYKTLDQAIMKFHSMKMEEINKIIRDLWRSTYRGQDIEYIEIRSDADENVSASDKRRNYNYRVV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Rad50 Antibody

Immunocytochemistry/ Immunofluorescence: Rad50 Antibody [NBP2-47316]

Immunocytochemistry/ Immunofluorescence: Rad50 Antibody [NBP2-47316]

Immunocytochemistry/Immunofluorescence: Rad50 Antibody [NBP2-47316] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Rad50 Antibody [NBP2-47316]

Immunohistochemistry-Paraffin: Rad50 Antibody [NBP2-47316]

Immunohistochemistry-Paraffin: Rad50 Antibody [NBP2-47316] - Staining of human rectum shows strong nuclear positivity in glandular cells.

Applications for Rad50 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Rad50

Rad50 is a 153kDa protein that associates with Mre11 and p95 (Nibrin) to form a multiprotein complex involved in the double strand break repair process. Upon DNA damage, the complex translocates to the nucleus of damaged cells, binds DNA, and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. Overall, Rad50 is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination.

Alternate Names

DNA repair protein RAD50, EC 3.6, EC 3.6.1.15, EC 3.6.3, EC 3.6.3.27, hRAD50, NBSLD, RAD50 (S. cerevisiae) homolog, RAD50 homolog (S. cerevisiae), RAD502, RAD50-2

Gene Symbol

RAD50

Additional Rad50 Products

Product Documents for Rad50 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Rad50 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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