Rad51 Antibody (14B4)
Novus Biologicals, part of Bio-Techne | Catalog # NB100-148
Key Product Details
Validated by
Knockout/Knockdown, Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat, Chicken
Cited:
Human, Mouse, Nematode - Caenorhabditis elegans
Applications
Validated:
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, In vitro assay, Knockdown Validated, Proximity Ligation Assay, Western Blot
Cited:
Chemotaxis, Chromatin Immunoprecipitation (ChIP), IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, In vitro assay, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 14B4
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Full length (amino acids 1-338) Rad51 expressed in E. coli.
Reactivity Notes
C. elegans reactivity reported in scientific literature (PMID: 23942865). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Rad51 Antibody (14B4)
Western Blot: Rad51 Antibody (14B4) [NB100-148]
Western Blot: Rad51 Antibody (14B4) [NB100-148] - eIF3f facilitates hMSH4 stabilization. Western blotting analysis of the levels of HDAC3, hRad51, and VBP1 expression in 293T, 293T/eIF3f, and 293T/eIF3f-hMSH4 cells. 293T/eIF3f-hMSH4 cells treated with 1 or 10 Gy IR were fractionated at 6 hrs post-treatment and the levels of hMSH4 and eIF3f in the nuclear and cytoplasmic fractions were determined by immunoblotting. Alpha-tubulin was used as a marker for the cytoplasmic fraction. Image collected and cropped by CiteAb from the following publication (https://molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-12-51) licensed under a CC-BY license.Immunocytochemistry/ Immunofluorescence: Rad51 Antibody (14B4) [NB100-148]
Immunocytochemistry/Immunofluorescence: Rad51 Antibody (14B4) [NB100-148] - HCC1937 (human immortalized breast cancer) cells treated with PARPi for 96 h. Nuclei are stained in blue (DAPI) and RAD51 in red. ICC/IF image submitted by a verified customer review.Immunohistochemistry-Paraffin: Rad51 Antibody (14B4) [NB100-148]
Immunohistochemistry-Paraffin: Rad51 Antibody (14B4) [NB100-148] - BT483 xenograft. RAD51 antibody 14B4 dilution at 1:200. Antigen Retrieval: Trilogy(TM) (EDTA based, pH 8.0) buffer, 15 min.Applications for Rad51 Antibody (14B4)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100 - 1:1000
Immunohistochemistry
1:100 - 1:1000
Immunohistochemistry-Paraffin
1:100 - 1:1000
Western Blot
1:500 - 1:3000
Application Notes
ChIP usage reported in scientific literature (PMID: 24023853). Rad51 antibody validated for IHC-Fr, ICC/IF from verified customer reviews. Use in In vitro assay reported in scientific literature (PMID: 27815389). PLA-Assay dependent.
Reviewed Applications
Read 5 reviews rated 4.8 using NB100-148 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol (pH7)
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Rad51
Alternate Names
BRCC5, hRAD51, HsRAD51, HsT16930, RAD51 (S. cerevisiae) homolog (E coli RecA homolog), RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae), RAD51 homolog A, RAD51ABRCA1/BRCA2-containing complex, subunit 5, RAD51L3, RecA, E. coli, homolog of, RECADNA repair protein RAD51 homolog 1, RecA-like protein, recombination protein A
Entrez Gene IDs
5888 (Human)
Gene Symbol
RAD51
UniProt
Additional Rad51 Products
Product Documents for Rad51 Antibody (14B4)
Product Specific Notices for Rad51 Antibody (14B4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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