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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # 523713

Concentration

1.0 mg/ml

Product Summary for Rad51 Antibody (523713) [Unconjugated]

Immunogen

E. coli-derived partial recombinant human Rad51 protein (amino acids 174-339)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Scientific Data Images for Rad51 Antibody (523713) [Unconjugated]

Immunocytochemistry/Immunofluorescence: Rad51 Antibody (523713) [Unconjugated] [MAB99481] - Rad51 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse Rad51 Monoclonal Antibody (Catalog # MAB99481) at 8 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei.

Applications for Rad51 Antibody (523713) [Unconjugated]

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

5 - 10 ug/ml

Immunohistochemistry

1:10 - 1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Rad51

Rad51 is involved in both the recombination and repair of DNA damage. Its function may be modulated by interaction with other repair proteins including BRCA1 and BRCA2. Alterations in the Rad51 gene have been associated with increased cancer risk. Rad51 is conserved across species and is homologous to prokaryotic RecA.

Alternate Names

BRCC5, hRAD51, HsRAD51, HsT16930, RAD51 (S. cerevisiae) homolog (E coli RecA homolog), RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae), RAD51 homolog A, RAD51ABRCA1/BRCA2-containing complex, subunit 5, RAD51L3, RecA, E. coli, homolog of, RECADNA repair protein RAD51 homolog 1, RecA-like protein, recombination protein A

Gene Symbol

RAD51

Additional Rad51 Products

Product Documents for Rad51 Antibody (523713) [Unconjugated]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rad51 Antibody (523713) [Unconjugated]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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