RAD51AP1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-1129
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Predicted:
Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-ARVKPLHPNATST corresponding to C-Terminus according to NP_001124334.1, NP_006470.
Specificity
This antibody is expected to recognise both reported isoforms.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for RAD51AP1 Antibody
Immunohistochemistry-Paraffin: RAD51AP1 Antibody [NB100-1129] -
Immunohistochemistry-Paraffin: RAD51AP1 Antibody [NB100-1129] - (4ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Flow Cytometry: RAD51AP1 Antibody [NB100-1129] -
Flow Cytometry: RAD51AP1 Antibody [NB100-1129] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunocytochemistry/Immunofluorescence: RAD51AP1 Antibody [NB100-1129] -
Immunocytochemistry/Immunofluorescence: RAD51AP1 Antibody [NB100-1129] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).Applications for RAD51AP1 Antibody
Application
Recommended Usage
Flow Cytometry
10 ug/ml
Immunocytochemistry/ Immunofluorescence
10 ug/ml
Immunohistochemistry
4 - 6 ug/ml
Immunohistochemistry-Paraffin
4 - 6 ug/ml
Peptide ELISA
Detection limit 1:32000
Application Notes
Use in Western blot reported in scientific literature (PMID: 26323318).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: RAD51AP1
Alternate Names
PIR51RAD51-interacting protein, RAD51 associated protein 1, RAD51-associated protein 1
Entrez Gene IDs
10635 (Human)
Gene Symbol
RAD51AP1
UniProt
Additional RAD51AP1 Products
Product Documents for RAD51AP1 Antibody
Product Specific Notices for RAD51AP1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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