Rad51C Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1127

Novus Biologicals, part of Bio-Techne
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NB100-1127
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Rad51C Antibodies »

Product Specifications

Immunogen

Peptide with sequence RGKTFRFEMQRDL-C corresponding to N-Terminus according to NP_002867.1, NP_478123.1.

Specificity

This antibody is expected to recognise all reported human isoforms.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Rad51C Antibody

Western Blot: Rad51C Antibody [NB100-1127]

Western Blot: Rad51C Antibody [NB100-1127]

Western Blot: Rad51C Antibody [NB100-1127] - Western blot analysis of Rad51C in HeLa lysate using NB100-1127 at 2 ug/ ml.
Western Blot: Rad51C Antibody [NB100-1127]

Western Blot: Rad51C Antibody [NB100-1127]

Western Blot: Rad51C Antibody [NB100-1127] - (2ug/ml) of HeLa lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.

Applications for Rad51C Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:10 - 1:500

Peptide ELISA

Detection limit 1:32000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 45 kDa band observed in HeLa lysate (predicted MW of 41 kDa band according to NP_478123).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Rad51C

The Rad51 DNA repair protein is a key component of the double-strand break repair pathway and is essential for mitotic and meiotic recombination and genome stability. Five human RAD51 homologs have been identified: XRCC2, XRCC3, RAD51B, RAD51C, and RAD51D. Each of these homologs interacts with one or more of the others, with all of the proteins involved in one complex or multiple smaller complexes.

Alternate Names

BROVCA3, DNA repair protein RAD51 homolog 3, FANCO, MGC104277, R51H3, RAD51 homolog C, RAD51 homolog C (S. cerevisiae), RAD51L2RAD51 (S. cerevisiae) homolog C, RAD51-like protein 2, yeast RAD51 homolog 3

Entrez Gene IDs

5889 (Human)

Gene Symbol

RAD51C

UniProt

NP_002867 (Human)

Additional Rad51C Products

Product Documents for Rad51C Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Rad51C Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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