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Rad51L1 Antibody (OTI7H4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-01220

Novus Biologicals, part of Bio-Techne
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NBP2-01220
Conjugate
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI7H4

Concentration

0.27 mg/ml

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 119-346 of human RAD51L1(NP_598193) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Rad51L1 Antibody (OTI7H4)

Western Blot: Rad51L1 Antibody (OTI7H4) [NBP2-01220]

Western Blot: Rad51L1 Antibody (OTI7H4) [NBP2-01220]

Western Blot: Rad51L1 Antibody (7H4) [NBP2-01220] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Rad51L1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Rad51L1.
Immunocytochemistry/Immunofluorescence: Rad51L1 Antibody (7H4) [NBP2-01220] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Rad51L1.
Flow Cytometry: Rad51L1 Antibody (OTI7H4) [NBP2-01220]

Flow Cytometry: Rad51L1 Antibody (OTI7H4) [NBP2-01220]

Flow Cytometry: Rad51L1 Antibody (7H4) [NBP2-01220] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Rad51L1 antibody, and then analyzed by flow cytometry.

Applications for Rad51L1 Antibody (OTI7H4)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.27 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Rad51L1

The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are evolutionarily conserved proteins essential for DNA repair by homologous recombination. This protein has been shown to form a stable heterodimer with the family member RAD51C, which further interacts with the other family members, such as RAD51, XRCC2, and XRCC3. Overexpression of this gene was found to cause cell cycle G1 delay and cell apoptosis, which suggested a role of this protein in sensing DNA damage. At least three alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq].

Alternate Names

DNA repair protein RAD51 homolog 2, MGC34245, R51H2REC2hREC2, RAD51 (S. cerevisiae)-like 1, RAD51 homolog B, RAD51B, RAD51-like 1 (S. cerevisiae), RAD51-like protein 1, RecA-like protein, recombination repair protein

Entrez Gene IDs

5890 (Human)

Gene Symbol

RAD51B

Additional Rad51L1 Products

Product Documents for Rad51L1 Antibody (OTI7H4)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rad51L1 Antibody (OTI7H4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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