RAD54B Antibody (4A7)

Novus Biologicals, part of Bio-Techne | Catalog # H00025788-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4A7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RAD54B Antibodies »

Product Specifications

Immunogen

RAD54B (AAH01965, 801 a.a. ~ 910 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. HIQFSVEELKNLFTLHESSDCVTHDLLDCECTGEEVHTGDSLEKFIVSRDCQLGPHHQKSNSLKPLSMSQLKQWKHFSGDHLNLTDPFLERITENVSFIFQNITTQATGT

Reactivity Notes

Human. Other species not tested.

Specificity

RAD54B - RAD54 homolog B (S. cerevisiae)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for RAD54B Antibody (4A7)

Western Blot: RAD54B Antibody (4A7) [H00025788-M01]

Western Blot: RAD54B Antibody (4A7) [H00025788-M01]

Western Blot: RAD54B Antibody (4A7) [H00025788-M01] - RAD54B monoclonal antibody (M01), clone 4A7 Analysis of RAD54B expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: RAD54B Antibody (4A7) [H00025788-M01]

Immunocytochemistry/ Immunofluorescence: RAD54B Antibody (4A7) [H00025788-M01]

Immunocytochemistry/Immunofluorescence: RAD54B Antibody (4A7) [H00025788-M01] - Analysis of monoclonal antibody to RAD54B on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: RAD54B Antibody (4A7) [H00025788-M01] - Detection limit for recombinant GST tagged RAD54B is approximately 0.03ng/ml as a capture antibody.

Applications for RAD54B Antibody (4A7)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RAD54B

The protein encoded by this gene belongs to the DEAD-like helicase superfamily. It shares similarity with Saccharomyces cerevisiae RAD54 and RDH54, both of which are involved in homologous recombination and repair of DNA. This protein binds to double-stranded DNA, and displays ATPase activity in the presence of DNA. This gene is highly expressed in testis and spleen, which suggests active roles in meiotic and mitotic recombination. Homozygous mutations of this gene were observed in primary lymphoma and colon cancer.

Alternate Names

DNA repair and recombination protein RAD54B, EC 3.6.1, EC 3.6.4.-, fibrinogen silencer binding protein, FSBP, RAD54 homolog B, RAD54 homolog B (S. cerevisiae), RDH54

Entrez Gene IDs

25788 (Human)

Gene Symbol

RAD54B

OMIM

604289 (Human)

UniProt

AAH01965 (Human)

Additional RAD54B Products

Product Documents for RAD54B Antibody (4A7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RAD54B Antibody (4A7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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