Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 93A535

Format

Azide and BSA Free

Concentration

0.5 mg/ml

View all Rad9 Antibodies »

Product Specifications

Immunogen

The antibody was raised against fusion protein of human full length Rad9.

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Rad9 Antibody (93A535) - Azide and BSA Free

Western Blot: Rad9 Antibody (93A535)Azide and BSA Free [NBP2-80924]

Western Blot: Rad9 Antibody (93A535)Azide and BSA Free [NBP2-80924]

Western Blot: Rad9 Antibody (93A535) - Azide and BSA Free [NBP2-80924] - Western blot analysis of RAD9 recombinant protein using RAD9 antibody at 2 ug/ml. Human tissue TMA was used for this test. Image from the standard format of this antibody.
Immunohistochemistry: Rad9 Antibody (93A535) - Azide and BSA Free [NBP2-80924]

Immunohistochemistry: Rad9 Antibody (93A535) - Azide and BSA Free [NBP2-80924]

Immunohistochemistry: Rad9 Antibody (93A535) - Azide and BSA Free [NBP2-80924] - Formalin-fixed, paraffin-embedded human prostate adenocarcinoma stained with RAD9 antibody at 5 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT

Applications for Rad9 Antibody (93A535) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500. Use reported in scientific literature (Kotmatsu et al)

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500. Use reported in scientific literature (Lee et al (2007))

Immunoprecipitation

1:10-1:500

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Rad9

Rad9 is a recently isolated human protein that is involved in the control of cell cycle checkpoint. Human Rad9 (hRad9) is homologue of the S. pombe Rad9. Six Rad proteins (Rad1, Rad3, Rad9, Rad17, Rad26, and Hus1) are involved in the early steps of DNA damage check point response in S. pombe. Recently, it has been shown that hRad9 interacts with Bcl-2 family proteins Bcl-2 and Bcl-XL, but not with BAX or BAD. Over-expression of hRad9 in mammalian cells leads to apoptosis, where as antisense Rad9 suppresses cell death induced by DNA damaging agents such as methanosulphonate. Human Rad9 undergoes complex post-translational modifications in undamaged cells and is hyperphosphorylated in response to DNA damage and makes ternary complex with human Rad1 and human HUS1 proteins. This may account for detection of multiple protein bands in some cases by western blot or immunoprecipitation analysis using anti-hRad9 antibody. HRad9 has an intrinsic 3-5 exonuclease activity suggesting that by interaction with DNA it may play a role in the damage-sensing step of the DNA damage checkpoint pathway.

Alternate Names

cell cycle checkpoint control protein RAD9A, DNA repair exonuclease rad9 homolog A, EC 3.1.11.2, hRAD9, RAD9, RAD9 (S. pombe) homolog, RAD9 homolog A (S. pombe)

Gene Symbol

RAD9A

Additional Rad9 Products

Product Documents for Rad9 Antibody (93A535) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Rad9 Antibody (93A535) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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