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Raf-1 Antibody (M208)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23358

Novus Biologicals, part of Bio-Techne
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NBP3-23358

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # M208

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Clone (M208) was generated from a sequence corresponding to amino acids in the N-terminal region of human Raf-1. This Raf-1 sequence has high homology to rat and mouse Raf-1. [UniProt# P04049]

Specificity

This antibody detects a 74 kDa protein corresponding to the molecular mass of Raf-1 on SDS-PAGE immunoblots of human A431 and Jurkat cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

74 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for Raf-1 Antibody (M208)

Raf-1 Antibody (M208)

Western Blot: Raf-1 Antibody (M208) [NBP3-23358] -

Western Blot: Raf-1 Antibody (M208) [NBP3-23358] - Western blot of human A431 (lane 1) and Jurkat (lane 2) cells probed with mouse monoclonal anti-Raf-1 (N-terminal) antibody at 1:500.

Applications for Raf-1 Antibody (M208)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Raf-1

Raf-1 is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. The activated Raf-1 can phosphorylate and activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the Serine/Threonine specific protein kinases ERK1 and ERK2. Downstream of Raf-1, activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation, and cell migration.

Long Name

v-raf-1Murine Leukemia Viral Oncogene Homolog 1

Alternate Names

c-Raf-1, Raf1, v-Raf-1

Gene Symbol

RAF1

Additional Raf-1 Products

Product Documents for Raf-1 Antibody (M208)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Raf-1 Antibody (M208)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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