Raf-1 [p Ser259] Antibody (SR2181)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21573

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-21573-100ul
NBP3-21573-50ul
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR2181

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Raf-1 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human phospho-Raf-1 [Ser259] (Uniprot #: P04049)

Modification

p Ser 259

Specificity

Detects endogenous levels of total phospho-Raf-1 [Ser259]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

73 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Raf-1 [p Ser259] Antibody (SR2181)

Western Blot: Raf-1 [p Ser 259] Antibody (SR2181) [NBP3-21573] -

Western Blot: Raf-1 [p Ser 259] Antibody (SR2181) [NBP3-21573] - Western blot analysis using NBP3-21573 in (1) 293T cell lysate; (2) 293T cell lysates treated with Okadaic Acid + Calyculin A.

Applications for Raf-1 [p Ser259] Antibody (SR2181)

Application
Recommended Usage

Immunohistochemistry

1:50-1:100

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Raf-1

Raf-1 is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. The activated Raf-1 can phosphorylate and activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the Serine/Threonine specific protein kinases ERK1 and ERK2. Downstream of Raf-1, activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation, and cell migration.

Long Name

v-raf-1Murine Leukemia Viral Oncogene Homolog 1

Alternate Names

c-Raf-1, Raf1, v-Raf-1

Gene Symbol

RAF1

Additional Raf-1 Products

Product Documents for Raf-1 [p Ser259] Antibody (SR2181)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Raf-1 [p Ser259] Antibody (SR2181)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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