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RAG2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43666

Novus Biologicals, part of Bio-Techne
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NBP2-43666

Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Bovine (91%), Porcine (90%), Rabbit (91%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human RAG2. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RAG2 Antibody

Western Blot: RAG2 Antibody [NBP2-43666]

Western Blot: RAG2 Antibody [NBP2-43666]

Western Blot: RAG2 Antibody [NBP2-43666] - Analysis of A. 50 ug rat thymus lysate/extract 7.5 % SDS-PAGE RAG2 antibody dilution: 1:1000.
Immunocytochemistry/ Immunofluorescence: RAG2 Antibody [NBP2-43666]

Immunocytochemistry/ Immunofluorescence: RAG2 Antibody [NBP2-43666]

Immunocytochemistry/Immunofluorescence: RAG2 Antibody [NBP2-43666] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RAG2 protein stained by RAG2 antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10um.
Immunohistochemistry-Paraffin: RAG2 Antibody [NBP2-43666]

Immunohistochemistry-Paraffin: RAG2 Antibody [NBP2-43666]

Immunohistochemistry-Paraffin: RAG2 Antibody [NBP2-43666] - DLD1 Xenograft, using RAG2 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Applications for RAG2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7)

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RAG2

Alternate Names

RAG-2, recombination activating gene 2, V(D)J recombination-activating protein 2

Gene Symbol

RAG2

Additional RAG2 Products

Product Documents for RAG2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RAG2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.