RAIDD/CRADD Antibody (23#)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-42110

Novus Biologicals, part of Bio-Techne
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NBP3-42110
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 23#

Concentration

1 mg/ml

View all CRADD Antibodies »

Product Specifications

Immunogen

Immunized with recombinant Human RAIDD/CRADD (Uniprot#: P78560; Met1-Leu140 (Uniprot#: P78560)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Applications for RAIDD/CRADD Antibody (23#)

Application
Recommended Usage

ELISA

0.05-2ug/ml

Immunocytochemistry/ Immunofluorescence

5-30ug/ml

Immunohistochemistry-Frozen

5-30ug/ml

Immunohistochemistry-Paraffin

5-30ug/ml

Western Blot

0.5-5ug/ml

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS, pH7.4, 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CRADD

Apoptosis is triggered by activation of initiator caspases upon complex-mediated clustering of the inactive zymogen, as occurs in the caspase-9-activating apoptosome complex. Activation of caspase-2 occurs in a complex that contains the death domain-containing protein PIDD, whose expression is induced by p53, and the adaptor protein RAIDD (1). RAIDD has a dual-domain structure similar to that of FADD. It has an NH2-terminal caspase homology domain that interacts with caspase-2 and a COOH-terminal DD that interacts with RIP. RAIDD is constitutively expressed in many tissues and thus could play a role in regulating apoptosis in mammalian cells (2). In metazoan cells there exists inactive polypeptide precursors (zymogens), each composed of a prodomain, which is cleaved to activate the protease, and a large and small catalytic subunit. The coupling of these proteases to signaling pathways is probably mediated by adaptor molecules that contain protein-protein interaction motifs such as the death domain (3).

Long Name

CASP2 and RIPK1 Domain Containing Adaptor With Death Domain

Alternate Names

RAIDD

Gene Symbol

CRADD

Additional CRADD Products

Product Documents for RAIDD/CRADD Antibody (23#)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RAIDD/CRADD Antibody (23#)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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