RALY Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-97634
![Western Blot: RALY Antibody [NBP2-97634]](https://resources.bio-techne.com/images/products/RALY-Antibody-Western-Blot-NBP2-97634-img0004.jpg "Western Blot: RALY Antibody [NBP2-97634]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for RALY Antibody
Immunogen
Produced in rabbits immunized with E. coli-derived Human RALY fragment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for RALY Antibody
Western Blot: RALY Antibody [NBP2-97634]
Western Blot: RALY Antibody [NBP2-97634] - Anti-RALY rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: Jurkat Whole Cell Lysate Lane D: A549 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 32 kDa. Observed band size: 37 kDa![Immunocytochemistry/ Immunofluorescence: RALY Antibody [NBP2-97634]](https://resources.bio-techne.com/images/products/RALY-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-97634-img0002.jpg "Immunocytochemistry/ Immunofluorescence: RALY Antibody [NBP2-97634]")
Immunocytochemistry/ Immunofluorescence: RALY Antibody [NBP2-97634]
Immunocytochemistry/Immunofluorescence: RALY Antibody [NBP2-97634] - Immunofluorescence staining of RALY in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human RALY polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus.![Immunoprecipitation: RALY Antibody [NBP2-97634]](https://resources.bio-techne.com/images/products/RALY-Antibody-Immunoprecipitation-NBP2-97634-img0005.jpg "Immunoprecipitation: RALY Antibody [NBP2-97634]")
Immunoprecipitation: RALY Antibody [NBP2-97634]
Immunoprecipitation: RALY Antibody [NBP2-97634] - RALY was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 4 ul anti-RALY rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-RALY rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 32 kDa. Observed band size: 40 kDaApplications for RALY Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Immunoprecipitation
5-10 uL/mg of lysate
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: RALY
Alternate Names
Autoantigen p542, Heterogeneous nuclear ribonucleoprotein C-like 2, hnRNP associated with lethal yellow protein homolog, hnRNP core protein C-like 2, HNRPCL2MGC117312, P542hnRNP-associated with lethal yellow), RNA binding protein (autoantigenic, hnRNP-associated with lethal yellow), RNA binding protein, autoantigenic (hnRNP-associated with lethal yellow homolog(mouse)), RNA-binding protein (autoantigenic), RNA-binding protein Raly
Gene Symbol
RALY
Additional RALY Products
Product Specific Notices for RALY Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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