RanGAP1 Antibody (OTI1B4) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73797

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1B4

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all RanGAP1 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human RANGAP1 (NP_002874) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

63.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RanGAP1 Antibody (OTI1B4) - Azide and BSA Free

Western Blot: RanGAP1 Antibody (OTI1B4)Azide and BSA Free [NBP2-73797]

Western Blot: RanGAP1 Antibody (OTI1B4)Azide and BSA Free [NBP2-73797]

Western Blot: RanGAP1 Antibody (OTI1B4) - Azide and BSA Free [NBP2-73797] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RanGAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RanGAP1.
Immunocytochemistry/ Immunofluorescence: RanGAP1 Antibody (OTI1B4) - Azide and BSA Free [NBP2-73797]

Immunocytochemistry/ Immunofluorescence: RanGAP1 Antibody (OTI1B4) - Azide and BSA Free [NBP2-73797]

Immunocytochemistry/Immunofluorescence: RanGAP1 Antibody (OTI1B4) - Azide and BSA Free [NBP2-73797] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY RanGAP1.
Immunohistochemistry: RanGAP1 Antibody (OTI1B4) - Azide and BSA Free [NBP2-73797]

Immunohistochemistry: RanGAP1 Antibody (OTI1B4) - Azide and BSA Free [NBP2-73797]

Immunohistochemistry: RanGAP1 Antibody (OTI1B4) - Azide and BSA Free [NBP2-73797] - Staining of paraffin-embedded Human liver tissue using anti-RanGAP1 mouse monoclonal antibody.
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Applications for RanGAP1 Antibody (OTI1B4) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Western Blot

1:1000-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RanGAP1

RanGAP1, is a homodimeric 65-kD polypeptide that specifically induces the GTPase activity of RAN, but not of RAS by over 1,000-fold. RanGAP1 is the immediate antagonist of RCC1, a regulator molecule that keeps RAN in the active, GTP-bound state. The RANGAP1 gene encodes a 587-amino acid polypeptide. The sequence is unrelated to that of GTPase activators for other RAS-related proteins, but is 88% identical to Fug1, the murine homolog of yeast Rna1p. RanGAP1 and RCC1 control RAN-dependent transport between the nucleus and cytoplasm. RanGAP1 is a key regulator of the RAN GTP/GDP cycle.

Long Name

Ran GTPase Activating Protein 1

Alternate Names

Fug1

Gene Symbol

RANGAP1

Additional RanGAP1 Products

Product Documents for RanGAP1 Antibody (OTI1B4) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RanGAP1 Antibody (OTI1B4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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