RAP1GAP Antibody (8V9I6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16234

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16234-100ul
NBP3-16234-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8V9I6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all RAP1GAP Antibodies »

Product Summary for RAP1GAP Antibody (8V9I6)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Rap1GAP (P47736). MIEKMQGSRMDEQRCSFPPPLKTEEDYIPYPSVHEVLGREGPFPLILLPQFGGYWIEGTNHEITSIPETEPLQSPTTKVKLECNPTARIYRKHFLGKEHF

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RAP1GAP Antibody (8V9I6)

Western Blot: RAP1GAP Antibody (8V9I6) [NBP3-16234]

Western Blot: RAP1GAP Antibody (8V9I6) [NBP3-16234]

Western Blot: RAP1GAP Antibody (8V9I6) [NBP3-16234] - Western blot analysis of extracts of various cell lines, using RAP1GAP Rabbit mAb (NBP3-16234) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Immunocytochemistry/ Immunofluorescence: RAP1GAP Antibody (8V9I6) [NBP3-16234]

Immunocytochemistry/ Immunofluorescence: RAP1GAP Antibody (8V9I6) [NBP3-16234]

Immunocytochemistry/Immunofluorescence: RAP1GAP Antibody (8V9I6) [NBP3-16234] - Immunofluorescence analysis of NIH-3T3 cells using RAP1GAP Rabbit mAb (NBP3-16234) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for RAP1GAP Antibody (8V9I6)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RAP1GAP

Rap1GAP, an 85/95kDa protein, is a member of a family of GAP's specific for Rap1 (1). Though to be an important tumor suppressor, Rap1GAP has no sequence homology with other GAPs such as RasGAP, RanGAP and RhoGAP. Purified as a cytosolic and membrane-bound protein, both isoforms consist of two monomers each contain a dimerization and a catalytic domain (2-4). Mutations in the GAP domain of the tumor suppressor tuberin, can cause benign tumor phenotype tuberous sclerosis (5).

Alternate Names

GTPase activating protein 1, RAP1 GTPase activating protein

Gene Symbol

RAP1GAP

Additional RAP1GAP Products

Product Documents for RAP1GAP Antibody (8V9I6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RAP1GAP Antibody (8V9I6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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