RAP1GAP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13216

Novus Biologicals, part of Bio-Techne
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NBP3-13216
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Key Product Details

Species Reactivity

Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RAP1GAP Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of mouse RAP1GAP. The exact sequence is proprietary.

Reactivity Notes

Human (88%), Bovine (87%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for RAP1GAP Antibody

Western Blot: RAP1GAP Antibody [NBP3-13216]

Western Blot: RAP1GAP Antibody [NBP3-13216]

Western Blot: RAP1GAP Antibody [NBP3-13216] - Various tissue extracts (10 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RAP1GAP antibody (NBP3-13216) diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: RAP1GAP Antibody [NBP3-13216]

Immunocytochemistry/ Immunofluorescence: RAP1GAP Antibody [NBP3-13216]

Immunocytochemistry/Immunofluorescence: RAP1GAP Antibody [NBP3-13216] - RAP1GAP antibody detects RAP1GAP protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RAP1GAP stained by RAP1GAP antibody (NBP3-13216) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Frozen: RAP1GAP Antibody [NBP3-13216]

Immunohistochemistry-Frozen: RAP1GAP Antibody [NBP3-13216]

Immunohistochemistry-Frozen: RAP1GAP Antibody [NBP3-13216] - RAP1GAP antibody detects RAP1GAP protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: RAP1GAP protein stained by RAP1GAP antibody (NBP3-13216) diluted at 1:250. Red: NF-H, stained by NF-H antibody [GT114] diluted at 1:500. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 10 min
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Applications for RAP1GAP Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RAP1GAP

Rap1GAP, an 85/95kDa protein, is a member of a family of GAP's specific for Rap1 (1). Though to be an important tumor suppressor, Rap1GAP has no sequence homology with other GAPs such as RasGAP, RanGAP and RhoGAP. Purified as a cytosolic and membrane-bound protein, both isoforms consist of two monomers each contain a dimerization and a catalytic domain (2-4). Mutations in the GAP domain of the tumor suppressor tuberin, can cause benign tumor phenotype tuberous sclerosis (5).

Alternate Names

GTPase activating protein 1, RAP1 GTPase activating protein

Gene Symbol

RAP1GAP

Additional RAP1GAP Products

Product Documents for RAP1GAP Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RAP1GAP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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