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Rap2B Antibody (4F12-3C6)

Novus Biologicals, part of Bio-Techne | Catalog # H00005912-M01

Novus Biologicals, part of Bio-Techne
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H00005912-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 4F12-3C6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

RAP2B (AAH12362, 1 a.a. ~ 183 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MREYKVVVLGSGGVGKSALTVQFVTGSFIEKYDPTIEDFYRKEIEVDSSPSVLEILDTAGTEQFASMRDLYIKNGQGFILVYSLVNQQSFQDIKPMRDQIIRVKRYERVPMILVGNKVDLEGEREVSYGEGKALAEEWSCPFMETSAKNKASVDELFAEIVRQMNYAAQPNGDEGCCSACVIL

Localization

Cell membrane. Lipid-anchor; Cytoplasmic side.

Specificity

RAP2B - RAP2B, member of RAS oncogene family

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Rap2B Antibody (4F12-3C6)

Western Blot: Rap2B Antibody (4F12-3C6) [H00005912-M01]

Western Blot: Rap2B Antibody (4F12-3C6) [H00005912-M01]

Western Blot: Rap2B Antibody (4F12-3C6) [H00005912-M01] - RAP2B monoclonal antibody (M01), clone 4F12-3C6 Analysis of RAP2B expression in A-431.
Immunocytochemistry/ Immunofluorescence: Rap2B Antibody (4F12-3C6) [H00005912-M01]

Immunocytochemistry/ Immunofluorescence: Rap2B Antibody (4F12-3C6) [H00005912-M01]

Immunocytochemistry/Immunofluorescence: Rap2B Antibody (4F12-3C6) [H00005912-M01] - Analysis of monoclonal antibody to RAP2B on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: Rap2B Antibody (4F12-3C6) [H00005912-M01]

Immunohistochemistry-Paraffin: Rap2B Antibody (4F12-3C6) [H00005912-M01]

Immunohistochemistry-Paraffin: Rap2B Antibody (4F12-3C6) [H00005912-M01] - Analysis of monoclonal antibody to RAP2B on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B tissue. Antibody concentration 1 ug/ml.

Applications for Rap2B Antibody (4F12-3C6)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recominant protein and cell lysate for WB. It has also been used for IF, IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Rap2B

This intronless gene belongs to a family of RAS-related genes. The proteins encoded by these genes share approximately 50% amino acid identity with the classical RAS proteins and have numerous structural features in common. The most striking difference between the RAP and RAS proteins resides in their 61st amino acid: glutamine in RAS is replaced by threonine in RAP proteins. Evidence suggests that this protein may be polyisoprenylated and palmitoylated.

Long Name

Ras-related Protein 2B

Alternate Names

MGC20484, RAP2B, member of RAS oncogene family, Ras family small GTP binding protein RAP2B, ras-related protein Rap-2b, small GTP binding protein

Entrez Gene IDs

5912 (Human)

Gene Symbol

RAP2B

OMIM

179541 (Human)

Additional Rap2B Products

Product Documents for Rap2B Antibody (4F12-3C6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rap2B Antibody (4F12-3C6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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