RAP30 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-17973

Novus Biologicals, part of Bio-Techne
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NBP3-17973-0.1ml
NBP3-17973-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RAP30 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: YKPVANHQYNIEYERKKKEDGKRARADKQHVLDMLFSAFEKHQYYNLKDLVDITKQPVVYLKEILKEIGVQNVKGIHKNTWELKPEYRHYQGEEK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RAP30 Antibody

Western Blot: RAP30 Antibody [NBP3-17973]

Western Blot: RAP30 Antibody [NBP3-17973]

Western Blot: RAP30 Antibody [NBP3-17973] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-459
Immunocytochemistry/ Immunofluorescence: RAP30 Antibody [NBP3-17973]

Immunocytochemistry/ Immunofluorescence: RAP30 Antibody [NBP3-17973]

Immunocytochemistry/Immunofluorescence: RAP30 Antibody [NBP3-17973] - Staining of human cell line U-2 OS shows positivity in nucleus and cytoskeleton (microtubules).
Immunohistochemistry: RAP30 Antibody [NBP3-17973]

Immunohistochemistry: RAP30 Antibody [NBP3-17973]

Immunohistochemistry: RAP30 Antibody [NBP3-17973] - Staining of human rectum shows strong nuclear positivity in glandular cells.
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Applications for RAP30 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RAP30

Predicted to enable RNA polymerase II general transcription initiation factor activity. Involved in transcription by RNA polymerase II. Located in microtubule cytoskeleton and nucleoplasm. Part of transcription preinitiation complex.

Alternate Names

ATP-dependent helicase GTF2F2, BTF4, General transcription factor IIF 30 kDa subunit, general transcription factor IIF subunit 2, general transcription factor IIF, polypeptide 2, 30kDa, TF2F2, TFIIF, TFIIF-beta, Transcription initiation factor IIF subunit beta, Transcription initiation factor RAP30

Gene Symbol

GTF2F2

Additional RAP30 Products

Product Documents for RAP30 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RAP30 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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