RAP74 Antibody (OTI4B10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-17971

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Monkey, Primate

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4B10

Concentration

0.44 mg/ml

View all RAP74 Antibodies »

Product Summary for RAP74 Antibody (OTI4B10)

Immunogen

Full length human recombinant protein of human GTF2F1(NP_002087) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for RAP74 Antibody (OTI4B10)

Western Blot: RAP74 Antibody (OTI4B10) [NBP3-17971]

Western Blot: RAP74 Antibody (OTI4B10) [NBP3-17971]

Western Blot: RAP74 Antibody (OTI4B10) [NBP3-17971] - Analysis of extracts (35ug) from 9 different cell lines by using anti-RAP74 monoclonal antibody.
Immunohistochemistry-Paraffin: RAP74 Antibody (OTI4B10) [NBP3-17971]

Immunohistochemistry-Paraffin: RAP74 Antibody (OTI4B10) [NBP3-17971]

Immunohistochemistry-Paraffin: RAP74 Antibody (OTI4B10) [NBP3-17971] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-RAP74 mouse monoclonal antibody.
Flow Cytometry: RAP74 Antibody (OTI4B10) [NBP3-17971]

Flow Cytometry: RAP74 Antibody (OTI4B10) [NBP3-17971]

Flow Cytometry: RAP74 Antibody (OTI4B10) [NBP3-17971] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RAP74 antibody, and then analyzed by flow cytometry.
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Applications for RAP74 Antibody (OTI4B10)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.44 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RAP74

Enables several functions, including RNA polymerase II general transcription initiation factor activity; phosphatase activator activity; and promoter-specific chromatin binding activity. Involved in several processes, including positive regulation of transcription by RNA polymerase II; response to virus; and transcription initiation from RNA polymerase II promoter. Located in cell junction and nucleoplasm. Part of transcription factor TFIID complex.

Alternate Names

BTF4, General transcription factor IIF 74 kDa subunit, general transcription factor IIF subunit 1, general transcription factor IIF, polypeptide 1, 74kDa, TF2F1, TFIIF, TFIIF-alpha, Transcription initiation factor IIF subunit alpha, transcription initiation factor RAP74

Gene Symbol

GTF2F1

Additional RAP74 Products

Product Documents for RAP74 Antibody (OTI4B10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RAP74 Antibody (OTI4B10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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