Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all RAP80 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 650 and the C-terminus (residue 719) of human Receptor Associated Protein 80 using the numbering given in entry NP_057374.3 (GeneID 51720).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RAP80 Antibody

Western Blot: RAP80 Antibody [NB100-2347]

Western Blot: RAP80 Antibody [NB100-2347]

Western Blot: RAP80 Antibody [NB100-2347] - Detection of Human RAP80 on HeLa whole cell lysate. RAP80 was also immunoprecipitated using rabbit anti-RAP80 antibody NB100-2346.
Immunohistochemistry: RAP80 Antibody [NB100-2347]

Immunohistochemistry: RAP80 Antibody [NB100-2347]

Immunohistochemistry: RAP80 Antibody [NB100-2347] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- RAP80 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Applications for RAP80 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2500-1:10000
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RAP80

RAP80 is an ubiquitin binding protein containing 2 UIM (ubiquitin interacting motif) repeats. It is thought to recruit BRCA1 to DNA damage sites and work with BRCA1 in G2/M checkpoint control. RAP80 also interacts with retinoid-related testis-associated receptor and regulates estrogen receptor alpha. It can act as a transcriptional repressor and inhibits the interaction of NR6A1 with the corepressor NCOR1. At least four isoforms of RAP80 are known to exist.

Long Name

Receptor Associated Protein 80

Alternate Names

RXRIP110, UIMC1, X2HRIP110

Entrez Gene IDs

51720 (Human)

Gene Symbol

UIMC1

UniProt

Q96RL1 (Human)

Additional RAP80 Products

Product Documents for RAP80 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RAP80 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.