RAPGEF2 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-06549
![Western Blot: RAPGEF2 Antibody [NBP1-06549]](https://resources.bio-techne.com/images/products/RAPGEF2-Antibody-Western-Blot-NBP1-06549-img0014.jpg "Western Blot: RAPGEF2 Antibody [NBP1-06549]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Predicted:
Canine (100%), Mouse (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 975 and 1025 of human PDZ domain containing guanine nucleotide exchange factor (GEF) 1 (NP_055062.1).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for RAPGEF2 Antibody - BSA Free
Western Blot: RAPGEF2 Antibody [NBP1-06549]
Western Blot: RAPGEF2 Antibody [NBP1-06549] - Whole cell lysate (50 ug) from HeLa, Hep-G2, Jurkat, and GaMG cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PDZ-GEF1 antibodyused for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Immunohistochemistry-Paraffin: RAPGEF2 Antibody [NBP1-06549]](https://resources.bio-techne.com/images/products/RAPGEF2-Antibody-Immunohistochemistry-NBP1-06549-img0011.jpg "Immunohistochemistry-Paraffin: RAPGEF2 Antibody [NBP1-06549]")
Immunohistochemistry-Paraffin: RAPGEF2 Antibody [NBP1-06549]
Immunohistochemistry-Paraffin: RAPGEF2 Antibody [NBP1-06549] - Human ovarian carcinoma. Antibody: Affinity purified rabbit anti- PDZ-GEF1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB![Immunoprecipitation: RAPGEF2 Antibody [NBP1-06549]](https://resources.bio-techne.com/images/products/RAPGEF2-Antibody-Immunoprecipitation-NBP1-06549-img0012.jpg "Immunoprecipitation: RAPGEF2 Antibody [NBP1-06549]")
Immunoprecipitation: RAPGEF2 Antibody [NBP1-06549]
Immunoprecipitation: RAPGEF2 Antibody [NBP1-06549] - Detection of human PDZ-GEF1 by western blot of immunoprecipitates. Samples:Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PDZ-GEF1 antibody NBP1-06549 used for IP at 6 ug per reaction. For blotting immunoprecipitated PDZ-GEF1, NBP1-06549 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Applications for RAPGEF2 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:1000 - 1:5000
Immunoprecipitation
2 - 10 ug/mg lysate
Western Blot
1:1000 - 1:5000
Application Notes
Epitope retrieval with citrate buffer pH6.0 or Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: RAPGEF2
Alternate Names
CNrasGEF, KIAA0313, Neural RAP guanine nucleotide exchange protein, nRap GEP, NRAPGEP, PDZ domain containing guanine nucleotide exchange factor (GEF) 1, PDZGEF1, PDZ-GEF1PDZ domain-containing guanine nucleotide exchange factor 1, RA(Ras/Rap1A-associating)-GEF, RA-GEFDKFZP586O1422, Rap guanine nucleotide exchange factor (GEF) 2, rap guanine nucleotide exchange factor 2, Rap-GEP
Entrez Gene IDs
9693 (Human)
Gene Symbol
RAPGEF2
UniProt
Additional RAPGEF2 Products
Product Documents for RAPGEF2 Antibody - BSA Free
Product Specific Notices for RAPGEF2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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