RAPGEF3 Antibody (5A7K7)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16343
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 5A7K7
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for RAPGEF3 Antibody (5A7K7)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 824-923 of human RAPGEF3 (O95398). FIHEGNHTLVENLINFEKMRMMARAARMLHHCRSHNPVPLSPLRSRVSHLHEDSQVARISTCSEQSLSTRSPASTWAYVQQLKVIDNQRELSRLSRELEP
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for RAPGEF3 Antibody (5A7K7)
![Western Blot: RAPGEF3 Antibody (5A7K7) [NBP3-16343]](https://resources.bio-techne.com/images/products/RAPGEF3-Antibody-5A7K7-Western-Blot-NBP3-16343-img0002.jpg "Western Blot: RAPGEF3 Antibody (5A7K7) [NBP3-16343]")
Western Blot: RAPGEF3 Antibody (5A7K7) [NBP3-16343]
Western Blot: RAPGEF3 Antibody (5A7K7) [NBP3-16343] - Western blot analysis of extracts of various cell lines, using RAPGEF3 Rabbit mAb (NBP3-16343) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.![Immunohistochemistry-Paraffin: RAPGEF3 Antibody (5A7K7) [NBP3-16343]](https://resources.bio-techne.com/images/products/RAPGEF3-Antibody-5A7K7-Immunohistochemistry-Paraffin-NBP3-16343-img0001.jpg "Immunohistochemistry-Paraffin: RAPGEF3 Antibody (5A7K7) [NBP3-16343]")
Immunohistochemistry-Paraffin: RAPGEF3 Antibody (5A7K7) [NBP3-16343]
Immunohistochemistry-Paraffin: RAPGEF3 Antibody (5A7K7) [NBP3-16343] - Immunohistochemistry of paraffin-embedded human placenta using RAPGEF3 Rabbit mAb (NBP3-16343) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Applications for RAPGEF3 Antibody (5A7K7)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: RAPGEF3
Long Name
Rap guanine nucleotide exchange factor 3
Alternate Names
cAMP-GEFI, CGEF1, EPAC, EPAC 1, EPAC1
Gene Symbol
RAPGEF3
Additional RAPGEF3 Products
Product Documents for RAPGEF3 Antibody (5A7K7)
Product Specific Notices for RAPGEF3 Antibody (5A7K7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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