RAPGEF5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # H00009771-B01P

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Mouse IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RAPGEF5 Antibodies »

Product Specifications

Immunogen

RAPGEF5 (AAH39203, 1 a.a. - 444 a.a.) full-length human protein. MGSSRLRVFDPHLERKDSAAALSDRELPLPTFDVPYFKYIDEEDEDDEWSSRSQSSTEDDSVDSLLSDRYVVVSGTPEKILEHLLNDLHLEEVQDKETETLLDDFLLTYTVFMTTDDLCQALLRHYSAKKYQGKEENSDVPRRKRKVLHLVSQWIALYKDWLPEDEHSKMFLKTIYRNVLDDVYEYPILEKELKEFQKILGMHRRHTVDEYSPQKKNKALFHQFSLKENWLQHRGTVTETEEIFCHVYITEHSYVSVKAKVSSIAQEILLCSQLGKRVQLVKKFIKIAAHCKAQRNLNSFFAIVMGLNTASVSRLSQTWEKIPGKFRKLFSELESLTDPSLNHKAYRDAFKKMKPPKIPFVPLLLKDVTFIHEGNKTFLDNLVNFEKLHMIADTVRTLRHCRTNQFGDLSPKEHQELKSYVNHLYVIDSQQALFELSHRIEPRV

Specificity

RAPGEF5 - Rap guanine nucleotide exchange factor (GEF) 5,

Clonality

Polyclonal

Host

Mouse

Isotype

IgG

Scientific Data Images for RAPGEF5 Antibody

Western Blot: RAPGEF5 Antibody [H00009771-B01P]

Western Blot: RAPGEF5 Antibody [H00009771-B01P]

Western Blot: RAPGEF5 Antibody [H00009771-B01P] - Analysis of RAPGEF5 expression in transfected 293T cell line by RAPGEF5 polyclonal antibody. Lane 1: RAPGEF5 transfected lysate(48.95 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: RAPGEF5 Antibody [H00009771-B01P]

Immunocytochemistry/ Immunofluorescence: RAPGEF5 Antibody [H00009771-B01P]

Immunocytochemistry/Immunofluorescence: RAPGEF5 Antibody [H00009771-B01P] - Analysis of purified antibody to RAPGEF5 on HepG2 cell. (antibody concentration 10 ug/ml)

Applications for RAPGEF5 Antibody

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control. Has also been used for immunofluorescence.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RAPGEF5

Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RAPGEF5, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]).[supplied by OMIM]

Alternate Names

GFRREPAC, Guanine nucleotide exchange factor for Rap1, KIAA0277M-Ras-regulated GEF, M-Ras-regulated Rap GEF, MRGEF, MR-GEFrap guanine nucleotide exchange factor 5, Rap guanine nucleotide exchange factor (GEF) 5, Related to Epac, Repac

Entrez Gene IDs

9771 (Human)

Gene Symbol

RAPGEF5

UniProt

AAH39203 (Human)

Additional RAPGEF5 Products

Product Documents for RAPGEF5 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RAPGEF5 Antibody

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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