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RAPH1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90859

Novus Biologicals, part of Bio-Techne
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NBP1-90859
NBP1-90859-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (95%), Rat (95%). Backed by our 100% Guarantee.

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NPQPQQWSKMSVKKAPPPTRPKRNDSTRLTQAEISEQPTMATVVPQVPTSPKSSLSVQPGFLADLNRTLQRKSITRHGSLSSRMSRAEPTATMDDMALPPPPPELLSDQQKAGYGGSHISGYATLRRGPPPAPPKRDQNTKLSR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RAPH1 Antibody

Immunohistochemistry-Paraffin: RAPH1 Antibody [NBP1-90859]

Immunohistochemistry-Paraffin: RAPH1 Antibody [NBP1-90859]

Immunohistochemistry-Paraffin: RAPH1 Antibody [NBP1-90859] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
RAPH1 Antibody

Western Blot-RAPH1 Antibody [NBP1-90859] -

Western Blot-RAPH1 Antibody [NBP1-90859] - Analysis in human cell lines Caco-2 and HEK293 using Anti-RAPH1 antibody. Corresponding RAPH1 RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.

Applications for RAPH1 Antibody

Application
Recommended Usage

Flow Cytometry

Reactivity reported in (PMID: 25517094)

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Simple Western reported by internal validation: Tested in blood and purified protein from human plasma; separated by size; antibody dilution of 1:3000.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RAPH1

Alternate Names

candidate 18, candidate 9, Lamellipodin, PREL2, PREL-2, proline rich EVH1 ligand 2, Protein RMO1, Ras association (RalGDS/AF-6) and pleckstrin homology domains 1, ras-associated and pleckstrin homology domains-containing protein 1, RMO1

Gene Symbol

RAPH1

Additional RAPH1 Products

Product Documents for RAPH1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RAPH1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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