RAR alpha/NR1B1 Antibody (763)
Novus Biologicals, part of Bio-Techne | Catalog # NB200-322
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse
Predicted:
Bovine (100%), Canine (100%), Guinea Pig (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 763
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH. Accession # P10276
Reactivity Notes
Tested in Human. Mouse reactivity reported in scientific literature (PMID: 24504337) Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information. It is anticipated that the antibody will also work with bovine, canine, and guinea pig tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Specificity
Specific for endogenous levels of the ~48 kDa RAR-alpha isotype.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for RAR alpha/NR1B1 Antibody (763)
![Western Blot: RAR alpha/NR1B1 Antibody (763) [NB200-322]](https://resources.bio-techne.com/images/products/Retinoic-Acid-Receptor-alpha-Antibody-763-Western-Blot-NB200-322-img0002.jpg "Western Blot: RAR alpha/NR1B1 Antibody (763) [NB200-322]")
Western Blot: RAR alpha/NR1B1 Antibody (763) [NB200-322]
Western Blot: Retinoic Acid Receptor alpha Antibody (763) [NB200-322] - Analysis of hippocampal lysate showing specific immunolabeling of the ~48k RAR-a protein.Applications for RAR alpha/NR1B1 Antibody (763)
Application
Recommended Usage
Western Blot
1:1000
Application Notes
Western blot where a ~48 kDa band can be seen using rat brain hippocampal homogenate. (Tested in TBST (20 mM Tris/HCl (7.6), 137 mM NaCl, 1% Tween-20) containing 5% nonfat milk and 0.05% NaN3.) Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24504337). Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID:24504337).
Reviewed Applications
Read 1 review rated 3 using NB200-322 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: RAR alpha/NR1B1
Long Name
Retinoic Acid Receptor, alpha
Alternate Names
NR1B1, RARA
Gene Symbol
RARA
UniProt
Additional RAR alpha/NR1B1 Products
Product Documents for RAR alpha/NR1B1 Antibody (763)
Product Specific Notices for RAR alpha/NR1B1 Antibody (763)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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