RASGRP 4 Antibody (1F3)

Novus Biologicals, part of Bio-Techne | Catalog # H00115727-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1F3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RASGRP 4 Antibodies »

Product Specifications

Immunogen

RASGRP4 (NP_733748, 574 a.a. ~ 673 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. CGLCCHKHCRDQVKVECKKRPGAKGDAGPPGAPVPSTPAPHASCGSEENHSYTLSLEPETGCQLRHAWTQTESPHPSWETDTVPCPVMDPPSTASSKLDS

Reactivity Notes

Human. Other species not tested.

Specificity

RASGRP4 - RAS guanyl releasing protein 4

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for RASGRP 4 Antibody (1F3)

Immunohistochemistry-Paraffin: RASGRP 4 Antibody (1F3) [H00115727-M01]

Immunohistochemistry-Paraffin: RASGRP 4 Antibody (1F3) [H00115727-M01]

Immunohistochemistry-Paraffin: RASGRP 4 Antibody (1F3) [H00115727-M01] - Analysis of monoclonal antibody to RASGRP4 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.
Sandwich ELISA: RASGRP 4 Antibody (1F3) [H00115727-M01] - Detection limit for recombinant GST tagged RASGRP4 is approximately 0.3ng/ml as a capture antibody.

Applications for RASGRP 4 Antibody (1F3)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RASGRP 4

The protein encoded by this gene is a member of the Ras guanyl nucleotide-releasing protein (RasGRP) family of Ras guanine nucleotide exchange factors. It contains a Ras exchange motif, a diacylglycerol-binding domain, and two calcium-binding EF hands. This protein was shown to activate H-Ras in a cation-dependent manner in vitro. Expression of this protein in myeloid cell lines was found to be correlated with elevated level of activated RAS protein, and the RAS activation can be greatly enhanced by phorbol ester treatment, which suggested a role of this protein in diacylglycerol regulated cell signaling pathways. Studies of a mast cell leukemia cell line expressing substantial amounts of abnormal transcripts of this gene indicated that this gene may play an important role in the final stages of mast cell development.

Alternate Names

guanyl nucleotide releasing protein 4, RAS guanyl releasing protein 4, RAS guanyl-releasing protein 4

Entrez Gene IDs

115727 (Human)

Gene Symbol

RASGRP4

OMIM

607320 (Human)

Additional RASGRP 4 Products

Product Documents for RASGRP 4 Antibody (1F3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RASGRP 4 Antibody (1F3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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