RASGRP3 Antibody (OTI2C11) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73812
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # OTI2C11
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Human recombinant protein fragment corresponding to amino acids 380-690 of human RASGRP3 (NP_733772) produced in E.coli.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
78.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for RASGRP3 Antibody (OTI2C11) - Azide and BSA Free
Western Blot: RASGRP3 Antibody (OTI2C11)Azide and BSA Free [NBP2-73812]
Western Blot: RASGRP3 Antibody (OTI2C11) - Azide and BSA Free [NBP2-73812] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RASGRP3 .Immunohistochemistry: RASGRP3 Antibody (OTI2C11) - Azide and BSA Free [NBP2-73812]
Immunohistochemistry: RASGRP3 Antibody (OTI2C11) - Azide and BSA Free [NBP2-73812] - Analysis of Human lymphoma tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)Immunohistochemistry: RASGRP3 Antibody (OTI2C11) - Azide and BSA Free [NBP2-73812]
Immunohistochemistry: RASGRP3 Antibody (OTI2C11) - Azide and BSA Free [NBP2-73812] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)Applications for RASGRP3 Antibody (OTI2C11) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
1:150
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: RASGRP3
Alternate Names
Calcium and DAG-regulated guanine nucleotide exchange factor III, GRP3KIAA0846ras guanyl-releasing protein 3, Guanine nucleotide exchange factor for Rap1, RAS guanyl releasing protein 3 (calcium and DAG-regulated)
Gene Symbol
RASGRP3
Additional RASGRP3 Products
Product Documents for RASGRP3 Antibody (OTI2C11) - Azide and BSA Free
Product Specific Notices for RASGRP3 Antibody (OTI2C11) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...