RASSF1 Antibody (3F3) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-04342

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3F3

Format

BSA Free

Concentration

1.0 mg/ml

View all RASSF1 Antibodies »

Product Summary for RASSF1 Antibody (3F3) - BSA Free

Immunogen

Recombinant human RASSF1 (1-340 aa), purified from E. coli (NP_009113).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RASSF1 Antibody (3F3) - BSA Free

Western Blot: RASSF1 Antibody (3F3)BSA Free [NBP1-04342]

Western Blot: RASSF1 Antibody (3F3)BSA Free [NBP1-04342]

Western Blot: RASSF1 Antibody (3F3) [NBP1-04342] - Cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RASSF1A (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: 293T cell lysateLane 2.: RASSF1A transfected 293T cell lysate
Immunocytochemistry/ Immunofluorescence: RASSF1 Antibody (3F3) - BSA Free [NBP1-04342]

Immunocytochemistry/ Immunofluorescence: RASSF1 Antibody (3F3) - BSA Free [NBP1-04342]

Immunocytochemistry/Immunofluorescence: RASSF1 Antibody (3F3) [NBP1-04342] - Analysis of RASSF1A in A431 cells. The cell was stained at (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
Western Blot: RASSF1 Antibody (3F3)BSA Free [NBP1-04342]

Western Blot: RASSF1 Antibody (3F3)BSA Free [NBP1-04342]

Western Blot: RASSF1 Antibody (3F3) [NBP1-04342] - Cell lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RASSF1A (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysate
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Applications for RASSF1 Antibody (3F3) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RASSF1

The tumor suppressor gene RASSF1A (3p21.3), human Ras association domain family 1A, is expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, small intestine, colon, and peripheral blood leukocytes. RASSF1A expression is inhibited by hypermethylation of the CpG island including the promoter and early transcribed regions of the human RASSF1A gene. Hypermethylated RASSF1A promoter is observed in primary tumors. Furthermore, RASSF1A is related with cell cycle regulation, apoptosis and microtubule stability. Agathanggelou A. et al

Alternate Names

123F2, NORE2A, pancreas-specific ras association domain family 1 protein, Ras association (RalGDS/AF-6) domain family member 1, ras association domain-containing protein 1, RASSF1A, RDA32cardiac-specific ras association domain family 1 protein, REH3P21, tumor suppressor protein RDA32, WUGSC:H_LUCA12.5

Entrez Gene IDs

11186 (Human)

Gene Symbol

RASSF1

UniProt

Q9NS23 (Human)

Additional RASSF1 Products

Product Documents for RASSF1 Antibody (3F3) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for RASSF1 Antibody (3F3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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