RASSF3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89425

Novus Biologicals, part of Bio-Techne
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NBP1-89425
NBP1-89425-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all RASSF3 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MSSGYSSLEEDAEDFFFTARTSFFRRAPQGKPRSGQQDVEKEKETHSYLSKEEIKEKVHKYNLAVTDKLKMTLNSNGIYTG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RASSF3 Antibody

Western Blot: RASSF3 Antibody [NBP1-89425]

Western Blot: RASSF3 Antibody [NBP1-89425]

Western Blot: RASSF3 Antibody [NBP1-89425] - Analysis in control (vector only transfected HEK293T lysate) and RASSF3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: RASSF3 Antibody [NBP1-89425]

Immunocytochemistry/ Immunofluorescence: RASSF3 Antibody [NBP1-89425]

Immunocytochemistry/Immunofluorescence: RASSF3 Antibody [NBP1-89425] - Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.
Immunohistochemistry-Paraffin: RASSF3 Antibody [NBP1-89425]

Immunohistochemistry-Paraffin: RASSF3 Antibody [NBP1-89425]

Immunohistochemistry-Paraffin: RASSF3 Antibody [NBP1-89425] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.

Applications for RASSF3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RASSF3

The RAS oncogene (MIM 190020) is mutated in nearly one-third of all human cancers. Members of the RAS superfamily are plasma membrane GTP-binding proteins that modulate intracellular signal transduction pathways. A subfamily of RAS effectors, including RASSF3, share a RAS association (RA) domain.

Alternate Names

MGC119194, MGC119195, MGC119197, Ras association (RalGDS/AF-6) domain family 3, Ras association (RalGDS/AF-6) domain family member 3, ras association domain-containing protein 3, RASSF5

Gene Symbol

RASSF3

Additional RASSF3 Products

Product Documents for RASSF3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for RASSF3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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