Rat alpha2u-Globulin Antibody
R&D Systems, part of Bio-Techne | Catalog # AF586
Key Product Details
Species Reactivity
Rat
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Rat urine-derived alpha2u-Globulin
Specificity
Detects rat alpha2u-Globulin in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Rat alpha2u-Globulin Antibody
alpha2u-Globulin in Rat Liver.
a2u-Globulin was detected in perfusion fixed frozen sections of rat liver using 5 µg/mL Goat Anti-Rat a2u-Globulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF586) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Rat alpha2u-Globulin Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of rat liver
Sample: Perfusion fixed frozen sections of rat liver
Western Blot
0.1 µg/mL
Sample: Rat alpha2u-Globulin
Sample: Rat alpha2u-Globulin
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha 2u-Globulin
Rat alpha 2u-Globulin (AUG), also known as major urinary protein, belongs to the lipocalin protein family. Under complex hormonal control, alpha 2u-globulin is a low molecular weight protein expressed in a tissue-, age-, and sex-specific fashion. Circulating alpha 2u-globulin is expressed by the liver. It is removed from the plasma by the kidney and excreted.
Rodent studies have employed alpha 2u-globulin nephropathy as an experimental model for the development of kidney tumors. Induction of alpha 2u-globulin nephropathy alters the number, size and shape of hyaline droplets in the proximal tubules. Intra-cellular accumulation of alpha 2u-globulin induces cell death and necrosis of tubular epithelial cells.
Alternate Names
AUG, PGCL1
Additional alpha 2u-Globulin Products
Product Documents for Rat alpha2u-Globulin Antibody
Product Specific Notices for Rat alpha2u-Globulin Antibody
For research use only
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