Rat beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody

B3GAT1 is a key enzyme involved in human natural killer-1 (HNK-1) epitope synthesis. It adds a glucuronic residue to the terminal lactosamine residue (Gal beta1-4GlcNAc-) of a glycoprotein or glycolipid, which can be further sulfated to become the HNK-1 epitope, a unique trisaccharide structure, HSO₃-3GlcA beta1-3Gal beta1-4GlcNAc- (1, 2). The enzyme activity was found to be enhanced in the presence of sphingomyelin and phosphatidylinositol (3).The HNK-1 carbohydrate epitope is characteristically expressed on a series of cell adhesion molecules in addition to some glycolipids in the extracellular matrix and on the cell surface in the nervous system, where it is involved in cell‑cell and cell‑substratum interaction and recognition during the development of the nervous system (4). Like most known glycosyltransferases, B3GAT1 is a type II Golgi-resident transmembrane protein with a short N‑terminal cytoplasmic domain and a single‑pass transmembrane domain followed by an enzymatic domain in the lumen of Golgi apparatus. The enzyme activity was assayed using a phosphatase-coupled method (5).