Rat CD19 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7489
Key Product Details
Species Reactivity
Rat
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 771404
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant rat CD19
Met1-Gly287
Accession # NP_001013255
Met1-Gly287
Accession # NP_001013255
Specificity
Detects rat CD19 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant
human CD19 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Rat CD19 Antibody
Detection of Rat CD19 by Western Blot.
Western blot shows lysates of rat splenocytes. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Rat CD19 Monoclonal Antibody (Catalog # MAB7489) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for CD19 at approximately 60-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
CD19 in Rat Spleen.
CD19 was detected in perfusion fixed frozen sections of rat spleen using Mouse Anti-Rat CD19 Monoclonal Antibody (Catalog # MAB7489) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to B-cells inside the germinal layers. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.Applications for Rat CD19 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Perfusion fixed frozen sections of rat spleen
Sample: Perfusion fixed frozen sections of rat spleen
Western Blot
1 µg/mL
Sample: Rat splenocytes
Sample: Rat splenocytes
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD19
CD19 (also surface antigen B4 and Leu12) is a 95‑110 kDa member of the Immunoglobulin superfamily of molecules. It is expressed by B cells, and interacts with CD21 for the purpose of reducing the threshold of the antigen signal needed to with activate the BCR. CD19 ligation also promotes B cell:follicular dendritic cell (FDC) interaction and B cell proliferation in the FDC zone of the spleen. Mature rat CD19 is a 529 amino acid (aa) type I transmembrane glycoprotein (aa 19‑547). Based on mouse, it contains a 269 aa extracellular region (aa 19‑287) plus a 236 aa cytoplasmic domain. The extracellular region possesses two C2-type Ig-like domains (aa 20‑113 and 171‑271) and one utilized phosphorylation site at Ser225. The cytoplasmic domain contains five potential Tyr phosphorylation sites. There is one splice form that shows a two aa substitution after Gly489. Over aa 19‑287, rat CD19 shares 88% and 57% aa sequence identity with mouse and human CD19, respectively.
Alternate Names
CD19, CVID3, Leu-12
Entrez Gene IDs
Gene Symbol
CD19
UniProt
Additional CD19 Products
Product Documents for Rat CD19 Antibody
Product Specific Notices for Rat CD19 Antibody
For research use only
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