Rat CD34 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4117
Key Product Details
Species Reactivity
Validated:
Rat
Cited:
Mouse, Rat
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat CD34
Glu37-Ile293
Accession # NP_001100672
Glu37-Ile293
Accession # NP_001100672
Specificity
Detects rat CD34 in direct ELISAs and Western blots. In these formats, less than 1% cross-reactivity with recombinant porcine CD34 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Rat CD34 Antibody
CD34 in Rat Liver.
CD34 was detected in perfusion fixed frozen sections of rat liver using 15 µg/mL Rat CD34 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4117) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Rat CD34 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of rat liver
Sample: Perfusion fixed frozen sections of rat liver
Western Blot
0.1 µg/mL
Sample: Recombinant Rat CD34
Sample: Recombinant Rat CD34
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 4 reviews rated 3.8 using AF4117 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD34
Rat CD34 is a 80-100 kDa type I transmembrane glycoprotein that belongs to the CD34/Podocalyxin family of sialomucins. It is a widely used marker of activated hematopoietic stem/progenitor cells and is also expressed on adult vascular endothelial cells. Mature rat CD34 is predicted to be a 352 amino acid (aa) protein with a 257 aa extracellular domain (ECD) (aa 35-291) and 74 aa cytoplasmic tail (aa 313-386). Over the region used as immunogen, rat CD34 shares 83% and 57% aa identity with mouse and human CD34, respectively.
Alternate Names
CD34, HPCA1
Gene Symbol
CD34
UniProt
Additional CD34 Products
Product Documents for Rat CD34 Antibody
Product Specific Notices for Rat CD34 Antibody
For research use only
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