Rat CNTF Antibody

R&D Systems, part of Bio-Techne | Catalog # AB-557-NA

R&D Systems, part of Bio-Techne
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AB-557-NA
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Key Product Details

Species Reactivity

Validated:

Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Neutralization, Western Blot

Cited:

Neutralization

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all CNTF Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant rat CNTF
Ala2-Met200
Accession # P20294

Specificity

Detects rat CNTF in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Rat CNTF Antibody

CNTF antibody in Rat Spinal Cord by Immunohistochemistry (IHC-Fr).

CNTF in Rat Spinal Cord.

CNTF was detected in perfusion fixed frozen sections of rat spinal cord using Goat Anti-Rat CNTF Polyclonal Antibody (Catalog # AB-557-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown); (CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of glial cells in the white matter. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Neuron Survival antibody in Response to CNTF and Neutralization by Rat CNTF Antibody.

Neuron Survival in Response to CNTF and Neutralization by Rat CNTF Antibody.

Recombinant Rat CNTF (557-NT) supports the survival of dorsal root ganglion neurons from E10 chick embryos in a dose-dependent manner (orange line), as measured by MTT staining. Neuron survival elicited by Recombinant Rat CNTF (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat CNTF Polyclonal Antibody (Catalog # AB-557-NA). The ND50 is typically 7-15 µg/mL.

Applications for Rat CNTF Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of rat spinal cord

Western Blot

1 µg/mL
Sample: Recombinant Rat CNTF (Catalog # 557-NT)

Neutralization

Measured by its ability to neutralize the effect of CNTF on dorsal root ganglion neurons from E10 chick embryos. Davies, A.M. (1989) in Neurotrophic Factor Bioassay Using Dissociated Neurons, Nerve Growth Factor. Rush, R.A. (eds): John Willey and Sons, Ltd. 95. The Neutralization Dose (ND50) is typically 7-15 µg/mL in the presence of 1 ng/mL Recombinant Rat CNTF.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Reconstitution

Reconstitute at 1 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CNTF

Ciliary neurotrophic factor (CNTF) is a polypeptide initially purified from chick embryo ocular tissue and identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Subsequent studies have demonstrated that CNTF is a survival factor for additional neuronal cell types including: dorsal root ganglion sensory neurons, sympathetic ganglion neurons, embryonic motor neurons, major pelvic ganglion neurons, and hippocampal neurons. CNTF has also been shown to prevent the degeneration of motor axons after axotomy. The cDNA for CNTF encodes a 200 amino acid residue polypeptide that lacks a signal sequence. CNTF is highly conserved across species and exhibits cross-species activities. Human and rat CNTF share approximately 83% homology in their protein sequence. CNTF is structurally related to IL-6, IL-11, LIF, and OSM. All of these four helix bundle cytokines share gp130 as a signal-transducing subunit in their receptor complexes.

Long Name

Ciliary Neurotrophic Factor

Alternate Names

ciliary neurotrophic factor, HCNTF

Entrez Gene IDs

1270 (Human); 12803 (Mouse); 25707 (Rat)

Gene Symbol

CNTF

UniProt

P20294

Additional CNTF Products

Product Documents for Rat CNTF Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Rat CNTF Antibody

For research use only

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