Rat CXCL1/GRO alpha/KC/CINC-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF-515-NA
Key Product Details
Species Reactivity
Validated:
Rat
Cited:
Rat
Applications
Validated:
Immunohistochemistry, Neutralization, Western Blot
Cited:
ELISA Development, Immunocytochemistry, Neutralization
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant rat CXCL1/GRO alpha/KC/CINC-1
Ala25-Lys96
Accession # P14095
Ala25-Lys96
Accession # P14095
Specificity
Detects rat CXCL1/GRO alpha/KC/CINC-1 in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant mouse (rm) KC is observed and less than 10% cross-reactivity with recombinant human (rh) GRO beta, rhGRO gamma, rmMIP-2, recombinant rat (rr) CINC-2 alpha and rrCINC-3 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Rat CXCL1/GRO alpha/KC/CINC-1 Antibody
Chemotaxis Induced by CXCL1/CINC-1 and Neutralization by Rat CXCL1/CINC-1 Antibody.
Recombinant Rat CXCL1/CINC-1 (Catalog # 515-CN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL1/CINC-1 (4 ng/mL) is neutralized (green line) by increasing concentrations of Rat CXCL1/CINC-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-515-NA). The ND50 is typically 0.15-0.75 µg/mL.
CXCL1/GRO alpha/KC/CINC-1 in Rat Liver.
CXCL1/GROa/KC/CINC-1 was detected in perfusion fixed paraffin-embedded sections of rat liver using 15 µg/mL Rat CXCL1/GROa/KC/CINC-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-515-NA) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
CXCL1/GRO alpha/KC/CINC‑1 in Rat Liver.
CXCL1/GROa/KC/CINC-1 was detected in perfusion fixed frozen sections of rat liver using Rat CXCL1/GROa/KC/CINC-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-515-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Rat CXCL1/GRO alpha/KC/CINC-1 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed paraffin-embedded sections of rat liver
Sample: Perfusion fixed paraffin-embedded sections of rat liver
Western Blot
0.1 µg/mL
Sample: Recombinant Rat CXCL1/GRO alpha/KC/CINC-1 (Catalog # 515-CN)
Sample: Recombinant Rat CXCL1/GRO alpha/KC/CINC-1 (Catalog # 515-CN)
Neutralization
Measured by its ability to neutralize CXCL1/GRO alpha/KC/CINC-1-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.15-0.75 µg/mL in the presence of 4 ng/mL Recombinant Rat CXCL1/GRO alpha/KC/CINC-1.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL1/GRO alpha/KC/CINC-1
Cytokine-induced neutrophil chemoattractant 1 (CINC-1) was originally purified from media conditioned by IL-1 beta stimulated rat kidney epithelioid cells (NRK-52E). On the basis of its protein and DNA sequences, CINC-1 is a member of the alpha (CXC) subfamily of chemokines. Three additional rat CXC chemokines (CINC-2 alpha, CINC-2 beta, CINC-3/MIP-2), sharing approximately 63-67% amino acid sequence identity with CINC-1, have been identified. The protein sequence of rat CINC-1 is also 68%, 71%, and 69% identical to that of human GRO-alpha, GRO-beta, and GRO-gamma, respectively. Based on their sequence homology, it has been suggested that CINCs are the rat counterpart of human GROs.
Rat CINC-1 cDNA encodes a 96 amino acid residue precursor protein from which the amino-terminal 24 amino acid residues are cleaved to generate the mature CINC-1. Similar to other alpha chemokines, rat CINCs are potent neutrophil attractants and activators and have been shown to play an important role in the infiltration of neutrophils into inflammatory sites in rats.
Alternate Names
CINC-1, CINC1, GRO alpha, KC, MGSA-alpha
Gene Symbol
CXCL1
UniProt
Additional CXCL1/GRO alpha/KC/CINC-1 Products
Product Documents for Rat CXCL1/GRO alpha/KC/CINC-1 Antibody
Product Specific Notices for Rat CXCL1/GRO alpha/KC/CINC-1 Antibody
For research use only
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