Rat CXCL3/CINC-2 alpha/ beta Antibody
R&D Systems, part of Bio-Techne | Catalog # AF517
Key Product Details
Species Reactivity
Rat
Applications
Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant rat CXCL3/CINC-2 alpha/ beta
Arg33-Leu100
Accession # NP_612531
Arg33-Leu100
Accession # NP_612531
Specificity
Detects rat CXCL3/CINC-2 alpha/ beta in direct ELISAs and Western blots. In direct ELISAs, almost 100% cross-reactivity with recombinant rat (rr) CINC-2 alpha is observed, approximately 50% cross-reactivity with recombinant mouse (rm) MIP-2 is observed, 35% cross‑reactivity with rmKC is observed, 15% cross-reactivity with rrCINC-1 is observed, and 10% cross-reactivity with recombinant human (rh) IL‑8, rhGRO alpha, rhGRO beta, and rhGRO gamma is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Rat CXCL3/CINC-2 alpha/ beta Antibody
Chemotaxis Induced by CXCL3/CINC‑2 beta and Neutral-ization by Rat CXCL3/ CINC‑2 alpha/ beta Antibody.
Recombinant Rat CXCL3/ CINC-2 beta (Catalog # 517-CB) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL3/ CINC-2 beta (0.05 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat CXCL3/ CINC-2a/ beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF517). The ND50 is typically 0.4-2.0 µg/mL.Applications for Rat CXCL3/CINC-2 alpha/ beta Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Rat CXCL3/CINC-2 alpha (Catalog # 516-CA)
Recombinant Rat CXCL3/CINC-2 beta (Catalog # 517-CB)
Sample: Recombinant Rat CXCL3/CINC-2 alpha (Catalog # 516-CA)
Recombinant Rat CXCL3/CINC-2 beta (Catalog # 517-CB)
Neutralization
Measured by its ability to neutralize CXCL3/CINC-2 beta-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 0.4-2.0 µg/mL in the presence of 0.05 µg/mL Recombinant Rat CXCL3/CINC-2 beta.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL3/GRO gamma/CINC-2/DCIP-1
Alternate Names
CINC-2, Dcip1, Gm1960, GRO gamma
Gene Symbol
CXCL3
UniProt
Additional CXCL3/GRO gamma/CINC-2/DCIP-1 Products
Product Documents for Rat CXCL3/CINC-2 alpha/ beta Antibody
Product Specific Notices for Rat CXCL3/CINC-2 alpha/ beta Antibody
For research use only
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