Rat CXCL7/Thymus Chemokine-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1116
Key Product Details
Species Reactivity
Rat
Applications
ELISA Capture (Matched Antibody Pair), Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 186701
Product Specifications
Immunogen
E. coli-derived recombinant rat CXCL7/Thymus Chemokine-1
Ile46-Ile107
Accession # NP_714943
Ile46-Ile107
Accession # NP_714943
Specificity
Detects rat CXCL7/Thymus Chemokine‑1 in ELISAs and Western blots. In sandwich ELISAs, no cross-reactivity or interference with recombinant human CXCL5, recombinant mouse CXCL7/Thymus Chemokine-1, recombinant rat (rr) CXCL1/CINC1, rrCXCL2/CINC3, rrCXCL3/CINC-2 alpha, rrCXCL3/CINC-2 beta, or rrCXCL5 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Rat CXCL7/Thymus Chemokine-1 Antibody
Chemotaxis Induced by CXCL7/Thymus Chemokine‑1 and Neutralization by Rat CXCL7/Thymus Chemokine‑1 Antibody.
Recombinant Rat CXCL7/Thymus Chemokine-1 (Catalog # 1116-CK) chemo-attracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CXCL7/ Thymus Chemokine-1 (15 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat CXCL7/ Thymus Chemokine-1 Monoclonal Antibody (Catalog # MAB1116). The ND50 is typically 4-20 µg/mL.Applications for Rat CXCL7/Thymus Chemokine-1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Rat CXCL7/Thymus Chemokine-1 (Catalog # 1116-CK)
Sample: Recombinant Rat CXCL7/Thymus Chemokine-1 (Catalog # 1116-CK)
Neutralization
Measured by its ability to neutralize CXCL7/Thymus Chemokine-1-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 4-20 µg/mL in the presence of 15 ng/mL Recombinant Rat CXCL7/Thymus Chemokine-1.
Rat Thymus Chemokine-1 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL7/Thymus Chemokine-1
CXCL7, also known as Thymus Chemokine-1, is a member of the alpha (CXC) subfamily of chemokines. It functions as a chemoattractant for neutrophils, macrophages and T cells that express CXCR2/IL-8 R beta. Rat CXCL7 cDNA encodes a 111 amino acid (aa) precursor protein. Rat CXCL7 shares 72% aa sequence identity with mouse CXCL7.
Alternate Names
Thymus Chemokine1
Gene Symbol
PPBP
UniProt
Additional CXCL7/Thymus Chemokine-1 Products
Product Documents for Rat CXCL7/Thymus Chemokine-1 Antibody
Product Specific Notices for Rat CXCL7/Thymus Chemokine-1 Antibody
For research use only
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