Rat Cystatin C Antibody

Name: Rat Cystatin C Antibody
Brand: R&D Systems
SKU: MAB6154

R&D Systems, part of Bio-Techne | Catalog # MAB6154

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Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Rat

Applications

Immunohistochemistry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2A Clone # 678920

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Product Summary for Rat Cystatin C Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat Cystatin C
Gly21-Ala140
Accession # P14841

Specificity

Detects rat Cystatin C in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Scientific Data Images for Rat Cystatin C Antibody

Cystatin C in Rat Endothelial Cells.

Cystatin C was detected in perfusion fixed frozen sections of rat brain using Mouse Anti-Rat Cystatin C Monoclonal Antibody (Catalog # MAB6154) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). Specific staining was localized to endothelial cells in vasculature. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Rat Cystatin C Antibody

Application

Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of rat brain

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Rat Cystatin C (Catalog # 6154‑PI), see our available Western blot detection antibodies

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cystatin C

Cystatin C is a member of family 2 in the Cystatin superfamily (1). It is involved in processes such as tumor invasion and metastasis, inflammation and some neurological diseases. It inhibits many cysteine proteases such as papain and cathepsins B, H, K, L and S (2, 3). It is ubiquitous in human tissues and body fluids. A point mutation in the gene coding for the 120 amino acid mature Cystatin C causes a hereditary form of amyloid angiopathy in which the protein variant is deposited in the cerebral arteries, leading to fatal cerebral hemorrhage (4). Cystatin C may have additional clinical applications. For example, it is a good marker for glomerular filtration rate (5).

References

Reed, C.H. (2000) British J. Biomed. Sci. 57:323.
Janowski, R. et al. (2001) Nat. Struct. Biol. 8:316.
Abrahamson, M. (1994) Methods Enzymol. 244:685.
Abrahamson, M. et al. (1992) Hum. Genet. 89:377.
Laterza, O.F. et al. (2002) Clin. Chem. 48:699.

Alternate Names

ARMD11, CST3, Gamma-trace, Neuroendocrine basic polypeptide, Post-gamma-globulin

Entrez Gene IDs

1471 (Human); 13010 (Mouse); 25307 (Rat)

Gene Symbol

CST3

UniProt

P14841

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Product Specific Notices for Rat Cystatin C Antibody

For research use only

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