Rat DLL1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3970

R&D Systems, part of Bio-Techne
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MAB3970
MAB3970-SP
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Key Product Details

Species Reactivity

Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 409005

View all DLL1 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat DLL1
Gln18-Trp537
Accession # P97677

Specificity

Detects rat DLL1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant rat (rr) Jagged-1, recombinant mouse (rm) Jagged-2, rmNotch‑3, rrNotch‑2, and rrNotch‑1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Rat DLL1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Rat DLL1 Fc Chimera (Catalog # 3970-DL)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DLL1

Delta-like protein 1 (DLL1) is a 90-100 kDa type I transmembrane protein in the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. Mature rat DLL1 consists of a 520 amino acid (aa) extracellular domain (ECD) with one DSL domain and eight EGF-like repeats, a 23 aa transmembrane segment, and a 154 aa cytoplasmic domain (1). Within the ECD, rat DLL1 shares 90% and 95% aa sequence identity with human and mouse DLL1, respectively. It shares 26%, 36%, and 53% aa sequence identity with rat DLL2, 3, and 4, respectively. The ADAM9, 12, or 17- mediated proteolysis of DLL1 releases a 60 kDa ECD fragment and regulates the Notch-dependent proliferation of hematopoietic and myogenic progenitor cells (2-4). The residual membrane-bound portion of DLL1 can be cleaved by presenilin-dependent gamma-secretase, enabling the cytoplasmic domain to migrate to the nucleus (5). DLL1 localizes to adherens junctions on neuronal processes through its association with the scaffolding protein MAGI1 (6). DLL1 is widely expressed, and it plays an important role in embryonic somite formation, cochlear hair cell differentiation, lymphocyte differentiation, and the maintenance of neural and myogenic progenitor cells (4, 7-13). The upregulation of DLL1 in arterial endothelial cells following injury or angiogenic stimulation is central to postnatal arteriogenesis (14). DLL1 is also overexpressed in cervical carcinoma and glioma and contributes to tumor progression (15-16).

References

  1. Bettenhausen, B. et al. (1995) Development 121:2407.
  2. Dyczynska, E. et al. (2007) J. Biol. Chem. 282:436.
  3. Karanu, F.N. et al. (2001) Blood 97:1960.
  4. Sun, D. et al. (2008) J. Cell Sci. 121:3815.
  5. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751.
  6. Mizuhara, E. et al. (2005) J. Biol. Chem. 280:26499.
  7. Takahashi, Y. et al. (2003) Development 130:4259.
  8. Teppner, I. et al. (2007) BMC Dev. Biol. 7:68.
  9. Kiernan, A.E. et al. (2005) Development 132:4353.
  10. Schmitt, T.M. and J.C. Zuniga-Pflucker (2002) Immunity 17:749.
  11. Hozumi, K. et al. (2004) Nat. Immunol. 5:638.
  12. Shimojo, H. et al. (2008) Neuron 58:52.
  13. Schuster-Gossler, K. et al. (2007) Proc. Natl. Acad. Sci. USA 104:537.
  14. Limbourg, A. et al. (2007) Circ. Res. 100:363.
  15. Purow, B.W. et al. (2005) Cancer Res. 65:2353.
  16. Gray, G.E. et al. (1999) Am. J. Pathol. 154:785.

Long Name

Delta-like 1

Alternate Names

Delta 1

Entrez Gene IDs

28514 (Human); 13388 (Mouse); 84010 (Rat)

Gene Symbol

DLL1

UniProt

P97677

Additional DLL1 Products

Product Documents for Rat DLL1 Antibody

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Product Specific Notices for Rat DLL1 Antibody

For research use only

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