Rat FGF-BP Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1413
Key Product Details
Species Reactivity
Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 227239
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat FGF-BP
Glu21-Cys238
Accession # Q9QY10
Glu21-Cys238
Accession # Q9QY10
Specificity
Detects rat FGF-BP in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human FGF-BP is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Applications for Rat FGF-BP Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Rat FGF-BP (Catalog # 1413-FB)
Sample: Recombinant Rat FGF-BP (Catalog # 1413-FB)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FGF-BP
Fibroblast growth factor binding protein (FGF-BP), also known as HBp17, is a secreted glycoprotein that increases the bioavailability of FGFs (1). Mature FGF-BP is a 34 kDa O-glycosylated protein with five conserved intrachain disulfide bonds (2‑4). FGF-BP contains a heparin-binding domain (aa 113‑146) and a distinct FGF-binding region (aa 197‑238) (5). Mature rat FGF-BP shares 54% and 81% aa sequence identity with mouse and rat FGF-BP, respectively. FGF-BP is expressed throughout development and in adult squamous epithelium (6, 7). It is upregulated in injured skin, renal tubular epithelium, and spinal nerves as well as in carcinomas of the skin, colon, and pancreas (3, 8‑11). FGF-BP binds FGF -1, -2, -7, -10, and -22 which are secreted and sequestered in the extracellular matrix (ECM) (8, 12). The interactions of FGF-BP with heparin sulfate proteoglycans (HSPG) and FGF, modulates their activities (7, 9 13, 14). FGF-BP enhances the mitogenic effects of FGFs, thereby contributing to epithelial, endothelial, and neuronal tissue repair, angiogenesis, and tumor growth (8‑10, 12, 15, 16).
References
- Abuharbeid, S. et al. (2006) Int. J. Biochem. Cell Biol. 38:1463.
- Aigner, A. et al. (2000) Growth Factors 18:51.
- Tassi, E. et al. (2006) Cancer Res. 66:1191.
- Lametsch, R. et al. (2000) J. Biol. Chem. 275:19469.
- Xie, B. et al. (2006) J. Biol. Chem. 281:1137.
- Aigner, A. et al. (2002) Histochem. Cell Biol. 117:1.
- Wu, D. et al. (1991) J. Biol. Chem. 266:16778.
- Beer, H.-D. et al. (2005) Oncogene 24:5269.
- Ray, P.E. et al. (2006) Am. J. Physiol. Regul. Integr. Comp. Physiol. 290:R105.
- Tassi, E. et al. (2007) Am. J. Physiol. Regul. Integr. Comp. Physiol. 293:R775.
- Kurtz, A. et al. (2004) Neoplasia 6:595.
- Tassi, E. et al. (2001) J. Biol. Chem. 276:40247.
- Mongiat, M. et al. (2001) J. Biol. Chem. 276:10263.
- Kurtz, A. et al. (1997) Oncogene 14:2671.
- Aigner, A. et al. (2001) Int. J. Cancer 92:510.
- Czubayko, F. et al. (1997) Nat. Med. 3:1137.
Long Name
Fibroblast Growth Factor Binding Protein
Alternate Names
FGF-BP1, FGFBP, FGFBP-1, FGFBP1, HBp17
Gene Symbol
FGFBP1
UniProt
Additional FGF-BP Products
Product Documents for Rat FGF-BP Antibody
Product Specific Notices for Rat FGF-BP Antibody
For research use only
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