Detects rat ICAM‑1/CD54 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) DCC, recombinant human (rh) ICAM-1, rmICAM-1, rmICAM-2, rhICAM-3, rmICAM-5, rmMAdCAM-1, rhCD31 or rmVCAM-1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Rat ICAM-1/CD54 Fluorescein-conjugated Antibody

Detection of ICAM‑1/CD54 in Rat Splenocytes by Flow Cytometry.

Rat splenocytes were stained with Mouse Anti-Rat ICAM-1/CD54 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB5831F, filled histogram) or isotype control antibody (Catalog # IC0041F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Rat ICAM-1/CD54 Fluorescein-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Rat splenocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: ICAM-1/CD54

Intercellular Adhesion Molecule-1 (ICAM-1, CD54), binds the leukocyte integrins LFA-1 ( alphaL/ beta2 or CD11a/CD18) and Mac-1 ( alphaM/ beta2 or CD11b/CD18). ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma, TNF-alpha, IL-1 beta, and LPS. Within the extracellular domain, rat ICAM-1 shares 53% and 78% amino acid sequence identity with human and mouse ICAM-1, respectively. Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, leukocyte adhesion deficiency syndrome (LAD), cancer, and transplantation.

References

Pigott, R. and C. Power (1993) in The Adhesion Molecule Facts Book, p. 74. Academic Press.
Siu, G. et al. (1989) J. Immunol. 143:3813.
Ballantyne, C.M. et al. (1989) Nuc. Acid. Res. 17:5853.

Long Name

Intercellular Adhesion Molecule 1

Alternate Names

CD54, ICAM1

Entrez Gene IDs

3383 (Human); 15894 (Mouse); 25464 (Rat)

Gene Symbol

ICAM1

UniProt

Q00238

Additional ICAM-1/CD54 Products

Product Documents for Rat ICAM-1/CD54 Fluorescein-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Rat ICAM-1/CD54 Fluorescein-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Rat ICAM-1/CD54 Fluorescein-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB5831F

Key Product Details

Species Reactivity

Validated:

Cited:

Applications