Rat IL-7R alpha/CD127 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5607
Key Product Details
Species Reactivity
Rat
Applications
CyTOF-ready, Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat IL-7 R alpha/CD127
Glu21-Asp239
Accession # NP_001099888
Glu21-Asp239
Accession # NP_001099888
Specificity
Detects rat IL-7 R alpha/CD127 in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross-reactivity with recombinant mouse IL-7 R alpha and less than 10% cross-reactivity with recombinant human IL-7 R alpha is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Rat IL-7R alpha/CD127 Antibody
Detection of Rat IL‑7 R alpha/CD127 by Western Blot.
Western blot shows lysates of rat spleen tissue. PVDF Membrane was probed with 1 µg/mL of Rat IL-7 Ra/CD127 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5607) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IL-7 Ra/CD127 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of IL‑7 R alpha/CD127 in Rat Splenocytes by Flow Cytometry.
Rat splenocytes were stained with Allophycocyanin-conjugated Mouse Anti-Rat CD4 Antibody and either (A) Normal Sheep IgG Control (Catalog # 5-001-A) or (B) Sheep Anti-Rat IL-7 Ra/CD127 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5607) followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126).Applications for Rat IL-7R alpha/CD127 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Rat splenocytes
Sample: Rat splenocytes
Western Blot
1 µg/mL
Sample: Rat spleen tissue
Sample: Rat spleen tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-7R alpha/CD127
IL-7 R alpha (IL-7 receptor alpha; also CD127) is a 60-70 kDa member of the type I cytokine receptor family of molecules. It is expressed on resting naïve and memory CD8+ T cells, DN thymocytes, pre-B cells, Th1 CD4+ T cells, dendritic cells and monocytes. IL-7 R alpha heterodimerizes with both the gammac chain to create the IL-7 receptor, and the TSLPR subunit to generate the TSLP receptor. IL-7 R alpha participates in T cell differentiation, naïve T cell survival, and CD4+ thymocyte proliferation. Mature rat IL-7 R alpha is a 437 aa type I transmembrane glycoprotein. It contains a 219 aa extracellular region (aa 21-239) that possesses one type III fibronectin domain (aa 129‑222), and a 193 aa cytoplasmic tail that shows a key phosphorylation site at Tyr447. Over aa 21‑239, rat IL-7 R alpha shares 79% and 67% aa identity with mouse and human IL-7 R alpha, respectively.
Long Name
Interleukin 7 Receptor alpha
Alternate Names
CD127, IL-7 R alpha, IL-7Ra, IL7R, IL7R alpha
Gene Symbol
IL7R
UniProt
Additional IL-7R alpha/CD127 Products
Product Documents for Rat IL-7R alpha/CD127 Antibody
Product Specific Notices for Rat IL-7R alpha/CD127 Antibody
For research use only
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