Rat Nestin Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2736
Key Product Details
Species Reactivity
Validated:
Rat
Cited:
Human, Mouse, Rat
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Western Blot
Cited:
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant rat Nestin
Met544-Glu820 (Gly756Asp, Ile758Met, Arg572Lys, Ala574Pro, Ile802Met, Arg816Lys)
Accession # EDM00749
Met544-Glu820 (Gly756Asp, Ile758Met, Arg572Lys, Ala574Pro, Ile802Met, Arg816Lys)
Accession # EDM00749
Specificity
Detects rat Nestin in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse Nestin is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Rat Nestin Antibody
Nestin in Rat Cortical Stem Cells.
Nestin was detected in immersion fixed rat cortical stem cells using 10 µg/mL Goat Anti-Rat Nestin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2736) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
beta‑III Tubulin and Nestin in Rat Cortical Stem Cells.
beta-III Tubulin and Nestin were detected in rat cortical stem cells (Catalog # NSC001) using 5 µg/mL neuron-specific Mouse beta-III Tubulin Monoclonal (clone TuJ-1) Antibody (Catalog # MAB1195) and 10 µg/mL Goat Anti-Rat Nestin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2736). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for beta-III Tubulin using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and for Nestin using the NorthernLights 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003). Tissue was counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Nestin in Rat Cortical Stem Cells.
Nestin was detected in immersion fixed rat cortical stem cells using Goat Anti-Rat Nestin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2736) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Rat Nestin Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed rat cortical stem cells and immersion fixed rat cortical neurons
Sample: Immersion fixed rat cortical stem cells and immersion fixed rat cortical neurons
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of rat embyo (E15)
Sample: Immersion fixed paraffin-embedded sections of rat embyo (E15)
Western Blot
0.1 µg/mL
Sample: Recombinant Rat Nestin
Sample: Recombinant Rat Nestin
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 4 reviews rated 4.5 using AF2736 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nestin
References
- Hockfield, S. and R.D. McKay (1985) J. Neurosci. 5:3310.
- Lendahl, U. et al. (1990) Cell 60:585.
Alternate Names
NES
Gene Symbol
NES
UniProt
Additional Nestin Products
Product Documents for Rat Nestin Antibody
Product Specific Notices for Rat Nestin Antibody
For research use only
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