Rat Neuropilin-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5661
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Rat
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 130604
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat Neuropilin-1
Met1-Asp854 (Lys811Arg, Pro812-Gly828 del), predicted
Accession # Q9QWJ9
Met1-Asp854 (Lys811Arg, Pro812-Gly828 del), predicted
Accession # Q9QWJ9
Specificity
Detects rat Neuropilin-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant rat Neuropilin-2 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Rat Neuropilin-1 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of rat embryo (E15)
Sample: Immersion fixed paraffin-embedded sections of rat embryo (E15)
Western Blot
1 µg/mL
Sample: Recombinant Rat Neuropilin-1/BDCA4 Fc Chimera (Catalog # 566-N1)
Sample: Recombinant Rat Neuropilin-1/BDCA4 Fc Chimera (Catalog # 566-N1)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Neuropilin-1
References
- Bielenberg, D.R. et al. (2006) Exp. Cell Res. 312:584.
- Gu, C. et al. (2003) Dev. Cell 5:45.
- He, Z. and M. Tessier-Lavigne (1997) Cell 90:739.
- Soker, S. et al. (1998) Cell 92:735.
- Gagnon, M.L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:2573.
- Cackowski, F.C. et al. (2004) Genomics 84:82.
- Rossignol, M. et al. (2000) Genomics 70:211.
- Gu, C. et al. (2002) J. Biol. Chem. 277:18069.
- Mamluk, R. et al. (2002) J. Biol. Chem. 277:24818.
- Yaron, A. et al. (2005) Neuron 45:513.
- Narazaki, M. and G. Tosato (2006) Blood 107:3892.
- Tordjman, R. et al. (2002) Nat. Immunol. 3:477.
Alternate Names
BDCA-4, CD304, Neuropilin1, NRP1
Gene Symbol
NRP1
UniProt
Additional Neuropilin-1 Products
Product Documents for Rat Neuropilin-1 Antibody
Product Specific Notices for Rat Neuropilin-1 Antibody
For research use only
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