Rat Nogo-A Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3098
Key Product Details
Species Reactivity
Rat
Applications
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 387521
Product Specifications
Immunogen
KLH-coupled rat Nogo-A synthetic peptide
SYDSIKLEPENPPPYEEA (aa 623-640)
Accession # Q9JK11
SYDSIKLEPENPPPYEEA (aa 623-640)
Accession # Q9JK11
Specificity
Detects rat Nogo-A in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Rat Nogo-A Antibody
Nogo‑A in Rat Embryo.
Nogo-A was detected in immersion fixed frozen sections of rat embryo (E18) using Mouse Anti-Rat Nogo-A Monoclonal Antibody (Catalog # MAB3098) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to spinal cord and dorsal root ganglia. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.Applications for Rat Nogo-A Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed frozen sections of rat embryo (E18)
Sample: Immersion fixed frozen sections of rat embryo (E18)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nogo-A
Nogo-A, also known as Reticulon-4, is the longest of four splice variants of Nogo. It is a CNS myelin-associated neurite outgrowth inhibitor that is highly expressed in oligodendrocytes. Nogo-A is synthesized as an 1163 amino acid protein and lacks a signal peptide. Within conserved C-terminal reticulon homology domain (RHD), two transmembrane domains, which are separated by a 66 amino acid extracellular loop, exist. Both the N-terminal domain and the 66 amino acid domain (Nogo-66) can be detected on the cell surface and show neurite outgrowth inhibitory activity. The amino acid sequence of rat Nogo-A N-terminal domain is 76% identical to that of human Nogo-A. Within the RHD domain, rat Nogo-A shares 99% and 97% amino acid sequence identity with mouse and human Nogo, respectively.
Long Name
Reticulon 4A
Alternate Names
NI220, NogoA, RTN4, RTN4A
Gene Symbol
RTN4
UniProt
Additional Nogo-A Products
Product Documents for Rat Nogo-A Antibody
Product Specific Notices for Rat Nogo-A Antibody
For research use only
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