Rat Reg3B Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1996
Key Product Details
Species Reactivity
Validated:
Rat
Cited:
Rat
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 293523
Product Specifications
Immunogen
E. coli-derived recombinant rat Reg3B
Glu27-Gly175
Accession # P25031
Glu27-Gly175
Accession # P25031
Specificity
Detects rat Reg3B in direct ELISAs and Western blots. Does not cross-react with recombinant mouse Reg1A, 1B, 2A, recombinant rat Reg3, or recombinant human Reg4.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Rat Reg3B Antibody
Reg3B in Rat Pancreas.
Reg3B was detected in perfusion fixed frozen sections of rat pancreas using Mouse Anti-Rat Reg3B Monoclonal Antibody (Catalog # MAB1996) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of exocrine cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Rat Reg3B Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Perfusion fixed frozen sections of rat pancreas (inflammatory conditions)
Sample: Perfusion fixed frozen sections of rat pancreas (inflammatory conditions)
Western Blot
1 µg/mL
Sample: Recombinant Rat Reg3B under non-reducing conditions only
Sample: Recombinant Rat Reg3B under non-reducing conditions only
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Reg3B
Long Name
Regenerating Islet-derived IIIb
Alternate Names
PAP1
Gene Symbol
REG3B
UniProt
Additional Reg3B Products
Product Documents for Rat Reg3B Antibody
Product Specific Notices for Rat Reg3B Antibody
For research use only
Loading...
Loading...
Loading...
Loading...