Rat Serpin A12 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5347

R&D Systems, part of Bio-Techne
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AF5347
AF5347-SP
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Key Product Details

Species Reactivity

Validated:

Rat

Cited:

Human

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all Serpin A12 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat Serpin A12
Leu20-Pro411
Accession # Q8R4Z1

Specificity

Detects rat Serpin A12 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross‑reactivity with recombinant human Serpin A12 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Rat Serpin A12 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Rat Serpin A12, see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Rat Serpin A12

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin A12

Serpin A12 (serine proteinase inhibitor-clade A #12; also vaspin) is a 45‑50 kDa, secreted member of the alpha1-antitrypsin (clade A)-subfamily, Serpin superfamily of protease inhibitors. It is produced by visceral and subcutaneous adipocytes, plus adipocyte-derived stem cells during obesity or diabetes. The presence of vaspin reverses insulin-resistance. Its molecular target is unknown, but it may antagonize the action of proteases that impair the action of insulin. Rat Serpin A12 is 411 amino acids (aa) in length. It contains a 20 aa signal sequence and a 391 aa mature region. A potential “active” inhibitor site lies between Gly364 and Pro389. Mature rat Serpin A12 shares 62% and 88% aa identity with human and mouse Serpin A12, respectively.

Alternate Names

OL-64, Vaspin

Entrez Gene IDs

145264 (Human); 68054 (Mouse); 191570 (Rat)

Gene Symbol

SERPINA12

UniProt

Q8R4Z1

Additional Serpin A12 Products

Product Documents for Rat Serpin A12 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Rat Serpin A12 Antibody

For research use only

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