Western blot shows lysates of NR8383 rat alveolar macrophage cell line and rat brain tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Rat SIRPa/CD172a Monoclonal Antibody (Catalog # MAB7307) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for SIRPa/CD172a at approximately 75-140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Adhesion Induced by SIRP alpha/ CD172a and Neutralization by Rat SIRP alpha/CD172a Antibody.

Recombinant Rat SIRPa/CD172a (Catalog # 7307-SA) supports the adhesion in mouse red blood cells in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Rat SIRPa/CD172a (6 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat SIRPa/CD172a Monoclonal Antibody (Catalog # MAB7307). The ND₅₀ is typically 0.04-0.16 µg/mL.

Detection of SIRP alpha/CD172a in PC‑12 Rat Cell Line by Flow Cytometry.

PC-12 rat adrenal pheochromocytoma cell line was stained with Mouse Anti-Rat SIRPa/CD172a Monoclonal Antibody (Catalog # MAB7307, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Applications for Rat SIRP alpha/CD172a Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: PC‑12 rat adrenal pheochromocytoma cell line

Western Blot

0.1 µg/mL
Sample: NR8383 rat alveolar macrophage cell line and rat brain tissue

Neutralization

Measured by its ability to neutralize SIRP alpha/CD172a-induced adhesion in mouse red blood cells. The Neutralization Dose (ND₅₀) is typically 0.04-0.16 μg/mL in the presence of 6 μg/mL Recombinant Rat SIRP alpha/CD172a.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SIRP alpha/CD172a

SIRP alpha (Signal regulatory protein alpha; also CD172a, Shps1 and Bit) is a variably glycosylated, 85-120 kDa member of the SIRP ‘family' of proteins. It is expressed on neurons, macrophages, monocytes, granulocytes and dendritic cells. SIRP alpha is phosphorylated/activated in response to cell adhesion. This may, or may not, involve binding to known ligands CD47, SP-A and SP-D. Following phosphorylation, SIRP alpha binds to SHP-1 and -2, resulting in the negative regulation of immune system activity. Mature rat SIRP alpha is a 478 amino acid (aa) type I transmembrane glycoprotein. It contains a 342 aa extracellular region (aa 32-373) that possesses one V-type and two C1-type Ig-like domains. Its cytoplasmic domain possesses two ITIMs that interact with protein phosphatases. There is one potential splice variant that shows a four aa insertion after Gln424. Over aa 32-373, rat SIRP alpha shares 63% and 73% aa sequence identity with human and mouse SIRP alpha, respectively.

Long Name

Signal-regulatory Protein alpha

Alternate Names

BIT, CD172a, MFR, MYD-1, SHPS1, SIRPA

Entrez Gene IDs

140885 (Human); 19261 (Mouse); 25528 (Rat); 494566 (Porcine); 609452 (Canine); 101926317 (Cynomolgus Monkey)

Gene Symbol

SIRPA

UniProt

P97710

Additional SIRP alpha/CD172a Products

Product Documents for Rat SIRP alpha/CD172a Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Rat SIRP alpha/CD172a Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Rat SIRP alpha/CD172a Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB7307

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Rat SIRP alpha/CD172a Antibody

Detection of Rat SIRP alpha/CD172a by Western Blot.